Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Fröhlich, R; Boehm, S; Illes, P.
Pharmacological characterization of P2 purinoceptor types in rat locus coeruleus neurons.
Eur J Pharmacol. 1996; 315(3):255-261
Doi: 10.1016/S0014-2999(96)00612-7
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- Co-Autor*innen der Med Uni Graz
- Böhm Stefan
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- Abstract:
- The frequency of spontaneous action potentials of locus coeruleus neurons was recorded extracellularly in pontine slices of the rat brain. The adenosine 5'-triphosphate (ATP) analogues alpha,beta-methylene ATP (alpha,beta-meATP) and 2-methylthio ATP increased the firing rate with a similar potency, while uridine 5'-triphosphate (UTP) was inactive. Diadenosine 5'-pentaphosphate (Ap5A), diadenosine 5'-tetraphosphate (Ap4A) and diadenosine 5'-triphosphate (Ap3A) all facilitated the firing. When equimolar concentrations were compared, Ap5A had the largest effect followed by Ap4A and Ap3A. Suramin markedly inhibited responses to alpha,beta-meATP and 2-methylthio ATP; the effect of Ap4A was only slightly depressed by suramin. Pyridoxalphosphate-6-azophenyl-2,4-disulfonic acid (PPADS) strongly antagonized alpha, beta-meATP, but failed to alter the effects of 2-methylthio ATP and Ap4A. Reactive blue 2 weakly antagonized alpha,beta-meATP and did not interfere with 2-methylthio ATP and Ap4A. Moreover, suramin depressed responses to (S)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartic acid (NMDA), but not to substance P. PPADS failed to affect the AMPA- and NMDA-induced increases in firing. Hence, locus coeruleus neurons may possess receptors for adenosine nucleotides (P2X and P2Y purinoceptors) and dinucleotides (P2D purinoceptors); receptors for uridine nucleotides (P2U purinoceptors or pyrimidinoceptors) are probably absent.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenosine - pharmacology
- Adenosine Triphosphate - pharmacology
- Animals -
- Dose-Response Relationship, Drug -
- Locus Coeruleus - drug effects
- Male -
- Membrane Potentials - drug effects
- Rats -
- Rats, Wistar -
- Receptors, Purinergic - drug effects
- Suramin - pharmacology
- Uridine Triphosphate - pharmacology
- Find related publications in this database (Keywords)
- purinoceptor
- pyrimidinoceptor
- excitatory amino acid receptor
- locus coeruleus
- extracellular recording