Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Boehm, S; Huck, S; Freissmuth, M.
Involvement of a phorbol ester-insensitive protein kinase C in the alpha2-adrenergic inhibition of voltage-gated calcium current in chick sympathetic neurons.
J Neurosci. 1996; 16(15):4596-4603
Doi: 10.1523/JNEUROSCI.16-15-04596.1996
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PubMed
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- Führende Autor*innen der Med Uni Graz
- Böhm Stefan
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- Abstract:
- alpha2-Adrenoceptors regulate the efficacy at the sympathoeffector junction by means of a feedback inhibition of transmitter release. In chick sympathetic neurons, the mechanism involves an inhibition of N-type calcium channels, and we now present evidence that this effect involves an atypical, phorbol ester-insensitive protein kinase C (PKC). The inhibition of voltage-gated Ca2+ currents by the specific alpha2-adrenergic agonist UK 14,304 was significantly attenuated when the PKC inhibitors PKC(19-36), staurosporine, or calphostin C were included in the internal solution used to fill the patch pipettes, or if staurosporine or calphostin C were applied extracellularly; however, phorbol esters as classical activators of PKC or oleoylacetylglycerol did not mimic the effect of UK 14,304, and chronic exposure to 4-beta-phorbol dibutyrate (PDBu) did not attenuate it, ever though PKCalpha and -epsilon isozymes were translocated to plasma membranes by PDBu. The atypical isozyme PKCzeta was translocated by 100 micrometer AA and this effect was attenuated when PKC(19-36) was added to the patch pipette solution. Our observations indicate that classical, new, and atypical PKC isozymes are present in chick sympathetic neurons and that an atypical, phorbol ester-insensitive PKC is involved in the inhibition of voltage-activated calcium currents by alpha2-adrenoceptor activation.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Calcium Channels - drug effects
- Cells, Cultured -
- Chickens -
- Dimethyl Sulfoxide - pharmacology
- Ganglia, Sympathetic - drug effects
- Patch-Clamp Techniques -
- Phorbol Esters - pharmacology
- Protein Kinase C - drug effects
- Receptors, Adrenergic, alpha-2 - drug effects
- Somatostatin - pharmacology
- Find related publications in this database (Keywords)
- alpha(2)-adrenoceptor
- calcium current
- chick sympathetic ganglion
- protein kinase C isozymes
- phorbol ester
- arachidonic acid