Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

Unterberger, U; Moskvina, E; Scholze, T; Freissmuth, M; Boehm, S.
Inhibition of adenylyl cyclase by neuronal P2Y receptors.
Br J Pharmacol. 2002; 135(3):673-684 Doi: 10.1038/sj.bjp.0704514 [OPEN ACCESS]
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Co-Autor*innen der Med Uni Graz: Böhm Stefan
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Abstract:: P2Y receptors inhibiting adenylyl cyclase have been found in blood platelets, glioma cells, and endothelial cells. In platelets and glioma cells, these receptors were identified as P2Y(12). Here, we have used PC12 cells to search for adenylyl cyclase inhibiting P2Y receptors in a neuronal cellular environment. ADP and ATP (0.1 - 100 microM) left basal cyclic AMP accumulation unaltered, but reduced cyclic AMP synthesis stimulated by activation of endogenous A(2A) or recombinant beta(2) receptors. Forskolin-dependent cyclic AMP production was reduced by ADPbetaS (71 nM)>ATP (164 nM)=ADP (244 nM). The inhibition by ADP was not antagonized by suramin, pyridoxal-phosphate-6-azophenyl-2',4'-disulphonic acid, or adenosine-3'-phosphate-5'-phosphate, but attenuated by reactive blue 2, ATP(alpha)S, and 2-methylthio-AMP. RT - PCR demonstrated the expression of P2Y(2), P2Y(4), P2Y(6), and P2Y(12), but not P2Y(1), receptors in PC12 cells. In Northern blots, only P2Y(2) and P2Y(12) were detectable. Differentiation with NGF did not alter these hybridization signals and left the nucleotide inhibition of adenylyl cyclase unchanged. We conclude that P2Y(12) receptors are expressed in neuronal cells and inhibit adenylyl cyclase activity.
Find related publications in this database (using NLM MeSH Indexing): Adenylate Cyclase - antagonists & inhibitors Adenylate Cyclase - metabolism; Animals -; Cyclic AMP - antagonists & inhibitors Cyclic AMP - metabolism; Dose-Response Relationship, Drug -; Membrane Proteins -; Neurons - enzymology Neurons - metabolism; PC12 Cells -; Rats -; Receptor, Adenosine A2A -; Receptors, Purinergic P1 - metabolism; Receptors, Purinergic P2 - physiology; Receptors, Purinergic P2Y12 -
Find related publications in this database (Keywords): adenylyl cyclase; ADP; ATP; neuronal P2Y receptor; PC12; pertussis toxin