Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bacsa, B; Kappe, CO.
Rapid solid-phase synthesis of a calmodulin-binding peptide using controlled microwave irradiation.
Nat Protoc. 2007; 2(9): 2222-2227.
Doi: 10.1038/nprot.2007.300
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Bacsa Bernadett
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- A rapid and efficient microwave-assisted solid-phase synthesis method for the preparation of a nonapeptide using conventional Fmoc/Bu(t) orthogonal protection strategy is described. In this protocol, the coupling steps are performed within 5 min at 60 degrees C and the Fmoc-deprotection steps are completed within 3 min at 60 degrees C using a dedicated single-mode microwave peptide synthesizer utilizing temperature-controlled conditions. It is demonstrated that the model nonapeptide (containing the calmodulin-binding octapeptide sequence) is synthesized in a shorter time (approximately 3.5 h) and with high purity (>95%) under microwave irradiation conditions in comparison with a reference peptide that is obtained by standard methods at room temperature (within 11 h).
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Binding Sites -
- Calmodulin - metabolism
- Combinatorial Chemistry Techniques -
- Microwaves -
- Peptides - chemical synthesis Peptides - chemistry Peptides - radiation effects