Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hatt, L; Brinch, M; Singh, R; Møller, K; Lauridsen, RH; Uldbjerg, N; Huppertz, B; Christensen, B; Kølvraa, S.
Characterization of fetal cells from the maternal circulation by microarray gene expression analysis--could the extravillous trophoblasts be a target for future cell-based non-invasive prenatal diagnosis?
Fetal Diagn Ther. 2014; 35(3):218-227
Doi: 10.1159/000356073
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Huppertz Berthold
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- Abstract:
- Circulating fetal cells in maternal blood provide a tool for risk-free, non-invasive prenatal diagnosis. However, fetal cells in the maternal circulation are scarce, and to effectively isolate enough of them for reliable diagnostics, it is crucial to know which fetal cell type(s) should be targeted. Fetal cells were enriched from maternal blood by magnetic-activated cell sorting using the endothelial cell marker CD105 and identified by XY fluorescence in situ hybridization. Expression pattern was compared between fetal cells and maternal blood cells using stem cell microarray analysis. 39 genes were identified as candidates for unique fetal cell markers. More than half of these are genes known to be expressed in the placenta, especially in extravillous trophoblasts (EVTs). Immunohistochemical staining of placental tissue confirmed CD105 staining in EVTs and 76% of fetal cells enriched by CD105 were found to be cytokeratin-positive. The unique combination of mesodermal (CD105) and ectodermal (cytokeratin) markers in EVTs could be a potential marker set for cell enrichment of this cell type in maternal blood and could be the basis for future cell-based non-invasive prenatal diagnosis. © 2013 S. Karger AG, Basel.
- Find related publications in this database (using NLM MeSH Indexing)
- Chorionic Villi - physiology
- Female -
- Gene Expression Regulation, Developmental -
- Humans -
- Male -
- Maternal Serum Screening Tests - methods
- Maternal-Fetal Exchange - physiology
- Pregnancy -
- Prenatal Diagnosis - methods
- Tissue Array Analysis - methods
- Trophoblasts - physiology
- Find related publications in this database (Keywords)
- Fetal cells in maternal blood
- Extravillous trophoblast
- Microarray
- Gene expression