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SHR Neuro Cancer Cardio Lipid Metab Microb
Bondarenko, AI; Jean-Quartier, C; Parichatikanond, W; Alam, MR; Waldeck-Weiermair, M; Malli, R; Graier, WF.
Mitochondrial Ca(2+) uniporter (MCU)-dependent and MCU-independent Ca(2+) channels coexist in the inner mitochondrial membrane.
Pflugers Arch. 2014; 466(7):1411-1420
Doi: 10.1007/s00424-013-1383-0
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- Leading authors Med Uni Graz
- Bondarenko Oleksandr
- Graier Wolfgang
- Co-authors Med Uni Graz
- Alam Muhammad Rizwan
- Jean-Quartier Claire
- Malli Roland
- Parichatikanond Warisara
- Waldeck-Weiermair Markus
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- Abstract:
- A protein referred to as CCDC109A and then renamed to mitochondrial calcium uniporter (MCU) has recently been shown to accomplish mitochondrial Ca(2+) uptake in different cell types. In this study, we investigated whole-mitoplast inward cation currents and single Ca(2+) channel activities in mitoplasts prepared from stable MCU knockdown HeLa cells using the patch-clamp technique. In whole-mitoplast configuration, diminution of MCU considerably reduced inward Ca(2+) and Na(+) currents. This was accompanied by a decrease in occurrence of single channel activity of the intermediate conductance mitochondrial Ca(2+) current (i-MCC). However, ablation of MCU yielded a compensatory 2.3-fold elevation in the occurrence of the extra large conductance mitochondrial Ca(2+) current (xl-MCC), while the occurrence of bursting currents (b-MCC) remained unaltered. These data reveal i-MCC as MCU-dependent current while xl-MCC and b-MCC seem to be rather MCU-independent, thus, pointing to the engagement of at least two molecularly distinct mitochondrial Ca(2+) channels.
- Find related publications in this database (using NLM MeSH Indexing)
- Action Potentials -
- Calcium - metabolism
- Calcium Channels - genetics Calcium Channels - metabolism
- HeLa Cells -
- Humans -
- Mitochondria - metabolism Mitochondria - physiology
- Mitochondrial Membranes - metabolism
- Sodium - metabolism
- Find related publications in this database (Keywords)
- Mitochondrial Ca2+ channels
- Mitochondrial Ca2+ uniporter
- MCU
- Ca2+ signaling