Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Reginato, E; Mroz, P; Chung, H; Kawakubo, M; Wolf, P; Hamblin, MR.
Photodynamic therapy plus regulatory T-cell depletion produces immunity against a mouse tumour that expresses a self-antigen.
Br J Cancer. 2013; 109(8):2167-2174
Doi: 10.1038/bjc.2013.580
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- Leading authors Med Uni Graz
- Reginato Eleonora
- Co-authors Med Uni Graz
- Wolf Peter
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- Abstract:
- Background: Photodynamic therapy (PDT) can lead to development of antigen-specific immune response and PDT-mediated immunity can be potentiated by T regulatory cell (Treg) depletion. We investigated whether the combination of PDT with cyclophosphamide (CY) could foster immunity against wild-type tumours expressing self-antigen (gp70). Methods: Mice with CT26 tumours were treated with PDT alone or in combination with low-dose CY. T regulatory cell numbers and transforming growth factor-beta (TGF-beta) levels were measured at several time points after treatment. Mice cured by PDT+CY were rechallenged with CT26 and monitored for long-term survival. Results: Photodynamic therapy+CY led to complete tumour regression and long-term survival in 90% of treated mice while the absolute numbers of Treg decreased after PDT+CY and the TGF-beta levels were reduced to a level comparable to naive mice. Sixty-five percent of the mice treated with PDT+CY that survived over 90 days tumour free rejected the rechallenge with the same tumour when a second dose of CY was administered before rechallenge but not without. Conclusion: Administration of CY before PDT led to depletion of Treg and potentiated PDT-mediated immunity, leading to long-term survival and development of memory immunity that was only uncovered by second Treg depletion.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antigens, Neoplasm - biosynthesis
- Autoantigens - biosynthesis
- Colonic Neoplasms - drug therapy
- Combined Modality Therapy -
- Cyclophosphamide - pharmacology
- Immunologic Memory - drug effects
- Immunosuppressive Agents - pharmacology
- Lymph Nodes - drug effects
- Mice -
- Mice, Inbred BALB C -
- Photochemotherapy - methods
- Spleen - drug effects
- T-Lymphocytes, Regulatory - drug effects
- Transforming Growth Factor beta - blood
- Find related publications in this database (Keywords)
- photodynamic therapy
- regulatory T cells
- TGF beta
- CT26 tumour
- anti-tumour immunity