Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Šinko, G; Vinković Vrček, I; Goessler, W; Leitinger, G; Dijanošić, A; Miljanić, S.
Alteration of cholinesterase activity as possible mechanism of silver nanoparticle toxicity.
Environ Sci Pollut Res Int. 2014; 21(2):1391-1400
Doi: 10.1007/s11356-013-2016-z
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PubMed
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- Co-authors Med Uni Graz
- Leitinger Gerd
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- Abstract:
- Due to their broad-spectrum antimicrobial activity, silver nanoparticles (AgNPs) have been used in a large number of commercial and medical products. Such proliferated AgNP production poses toxicological and environmental issues which need to be addressed. The present study aimed to investigate the effects of AgNPs on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), important enzymes in areas of neurobiology, toxicology and pharmacology. Three different AgNPs, prepared by the chemical reduction using trisodium citrate, hydroxylamine hydrochloride (Cl-AgNPs), and borohydride following stabilization with poly(vinyl alcohol), were purified and characterised with respect to their sizes, shapes and optical properties. Their inhibition potential on AChE and BChE was evaluated in vitro using an enzyme assay with o-nitrophenyl acetate or o-nitrophenyl butyrate as substrates, respectively. All three studied AgNPs were reversible inhibitors of ChEs. Among tested nanoparticles, Cl-AgNP was found to be the most potent inhibitor of both AChE and BChE. Although the detailed mechanism by which the AgNPs inhibit esterase activities remains unknown, structural perturbation of the enzyme may be the common mode of ChE inhibition by AgNPs.
- Find related publications in this database (using NLM MeSH Indexing)
- Acetylcholinesterase - metabolism
- Animals -
- Butyrylcholinesterase - metabolism
- Cholinesterase Inhibitors - toxicity
- Metal Nanoparticles - toxicity
- Models, Chemical -
- Risk Assessment -
- Silver - toxicity
- Find related publications in this database (Keywords)
- Silver nanoparticles
- Acetylcholinesterase
- Butyrylcholinesterase
- Inhibition