Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kump, PK; Gröchenig, HP; Lackner, S; Trajanoski, S; Reicht, G; Hoffmann, KM; Deutschmann, A; Wenzl, HH; Petritsch, W; Krejs, GJ; Gorkiewicz, G; Högenauer, C.
Alteration of intestinal dysbiosis by fecal microbiota transplantation does not induce remission in patients with chronic active ulcerative colitis.
Inflamm Bowel Dis. 2013; 19(10):2155-2165
Doi: 10.1097/MIB.0b013e31829ea325
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Constantini-Kump Patrizia
- Hoegenauer Christoph
- Co-authors Med Uni Graz
- Deutschmann Andrea
- Gorkiewicz Gregor
- Hoffmann Karl Martin
- Krejs Günter Josef
- Petritsch Wolfgang
- Trajanoski Slave
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- Abstract:
- In patients with ulcerative colitis (UC), alterations of the intestinal microbiota, termed dysbiosis, have been postulated to contribute to intestinal inflammation. Fecal microbiota transplantation (FMT) has been used as effective therapy for recurrent Clostridium difficile colitis also caused by dysbiosis. The aims of the present study were to investigate if patients with UC benefit from FMT and if dysbiosis can be reversed. Six patients with chronic active UC nonresponsive to standard medical therapy were treated with FMT by colonoscopic administration. Changes in the colonic microbiota were assessed by 16S rDNA-based microbial community profiling using high-throughput pyrosequencing from mucosal and stool samples. All patients experienced short-term clinical improvement within the first 2 weeks after FMT. However, none of the patients achieved clinical remission. Microbiota profiling showed differences in the modification of the intestinal microbiota between individual patients after FMT. In 3 patients, the colonic microbiota changed toward the donor microbiota; however, this did not correlate with clinical response. On phylum level, there was a significant reduction of Proteobacteria and an increase in Bacteroidetes after FMT. FMT by a single colonoscopic donor stool application is not effective in inducing remission in chronic active therapy-refractory UC. Changes in the composition of the intestinal microbiota were significant and resulted in a partial improvement of UC-associated dysbiosis. The results suggest that dysbiosis in UC is at least in part a secondary phenomenon induced by inflammation and diarrhea rather than being causative for inflammation in this disease.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Biological Therapy -
- Chronic Disease -
- Clostridium Infections - genetics
- Clostridium Infections - microbiology
- Clostridium Infections - therapy
- Colitis, Ulcerative - genetics
- Colitis, Ulcerative - microbiology
- Colitis, Ulcerative - therapy
- Dysbiosis - genetics
- Dysbiosis - microbiology
- Dysbiosis - prevention & control
- Feces - microbiology
- Female -
- Follow-Up Studies -
- Humans -
- Intestines - microbiology
- Male -
- Metagenome - genetics
- Microbiota -
- Middle Aged -
- Phylogeny -
- Prognosis -
- RNA, Ribosomal, 16S - analysis
- Remission Induction -
- Transplantation -
- Find related publications in this database (Keywords)
- inflammatory bowel disease
- ulcerative colitis
- fecal microbiota transplantation
- stool transplantation
- intestinal microbiota
- 16S rDNA
- microbial community profiling