Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Trescher, K; Hasun, M; Baumgartner, A; Dietl, W; Wolfsberger, M; Hallstrom, S; Podesser, BK; .
New HTK-N46B cardioplegia provides superior protection during ischemia/reperfusion in failing hearts.
J CARDIOVASC SURG. 2013; 54(3): 413-421.
Web of Science
PubMed
- Führende Autor*innen der Med Uni Graz
- Hallström Seth
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- Abstract:
- Aim. The aim of this paper was to improve operative outcome during open-heart surgery in patients with failing hearts, the composition of cardioplegic solutions has to be further optimized. HTK-N46b, a novel cardioplegic solution, has been developed for efficient protection of the energy state of myocytes as well as endothelial cells. Aim of this study is the evaluation of HTK-N46b in comparison to its precursor Custodiol (R) (HTK) in failing rat hearts undergoing ischemia/reperfusion. Methods. In male Sprague Dawley rats myocardial infarction (MI) was induced by LAD ligation. Six weeks after MI cardiac function was determined by transthoracic echocardiography. Sixteen animals with hearts showing a fractional shortening <25% were randomly assigned to two groups, HTK (N.=8) and HTK-N46b (N.=8). After excision hearts were evaluated in an erythrocyte-perfused isolated working heart model. Cold ischemia (4 degrees C) for 60 minutes was followed by 45 minutes of reperfusion. Cardiac arrest was induced either with HTK or HTK-N46b at the beginning of ischemia. Results. At similar preischemic fractional shortening (HTK-N46b: 14.41 +/- 1.83% vs. HTK: 14.91 +/- 1.92%; NS) postischemic recovery of stroke volume and stroke work were significantly improved in the HTK-N46b rat hearts compared to HTK. Concerning recovery of coronary flow there was no difference between groups. At the end of reperfusion the HTK-N46b protected group revealed higher levels of ATP (HTK-N46b: 22.01 +/- 0.89 nmol/mg protein vs. HTK: 16.83 +/- 1.72 nmol/mg protein; P<0.05) and energy charge (HTK-N46b: 0.82 +/- 0.02 vs. HTK: 0.74 +/- 0.02; P<0.05). Conclusion. HTK-N46b showed superior cardioprotective properties according to postischemic hemodynamic recovery and biochemical markers compared to HTK in failing rat hearts.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Biological Markers - metabolism
- Cardiac Surgical Procedures - methods
- Cardioplegic Solutions - pharmacology
- Disease Models, Animal -
- Glucose - pharmacology
- Heart Arrest, Induced - methods
- Heart Failure - complications Heart Failure - metabolism Heart Failure - surgery
- Male -
- Mannitol - pharmacology
- Myocardial Reperfusion Injury - etiology Myocardial Reperfusion Injury - metabolism Myocardial Reperfusion Injury - prevention & control
- Myocardium - metabolism
- Potassium Chloride - pharmacology
- Procaine - pharmacology
- Rats -
- Rats, Sprague-Dawley -
- Treatment Outcome -
- Find related publications in this database (Keywords)
- Heart arrest, induced
- Myocardial infarction
- Heart failure
- Models, animal