Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Oettl, K; Birner-Gruenberger, R; Spindelboeck, W; Stueger, HP; Dorn, L; Stadlbauer, V; Putz-Bankuti, C; Krisper, P; Graziadei, I; Vogel, W; Lackner, C; Stauber, RE.
Oxidative albumin damage in chronic liver failure: relation to albumin binding capacity, liver dysfunction and survival.
J Hepatol. 2013; 59(5):978-983
Doi: 10.1016/j.jhep.2013.06.013
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Öttl Karl
- Co-Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
- Krisper Peter
- Lackner Karoline
- Putz-Bankuti Csilla
- Spindelböck Walter Johann
- Stadlbauer-Köllner Vanessa
- Stauber Rudolf
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- Abstract:
- Impaired binding function of albumin has been demonstrated in end-stage liver disease. This and other functional disturbances of albumin may be related to oxidative stress which is believed to play an important role in the pathogenesis of liver failure as well as sepsis. The aim of the present study was to relate oxidative modification of albumin to loss of albumin binding function in advanced chronic liver failure and in sepsis. Patients with decompensated cirrhosis or sepsis and healthy controls were investigated. Three fractions of albumin were separated by chromatography according to the redox state of cysteine-34: non-oxidized human mercaptalbumin, reversibly oxidized human non-mercaptalbumin-1, and irreversibly oxidized human non-mercaptalbumin-2 (HNA2). Binding properties of albumin site II were measured using dansylsarcosine as a ligand. Both in cirrhotic and septic patients, fractions of oxidized albumin were increased and binding capacity for dansylsarcosine was decreased. Mass spectroscopy confirmed specific oxidation of cysteine-34. In cirrhotic patients, dansylsarcosine binding correlated strongly with liver function parameters and moderately with HNA2. Baseline levels of HNA2 accurately predicted 30-day and 90-day survival in cirrhotic patients and this was confirmed in an external validation cohort. Our results suggest that oxidative damage impairs binding properties of albumin. In advanced liver disease, reduced binding capacity of albumin site II is mainly related to impaired liver function. The plasma level of HNA2 is closely related to survival and may represent a novel biomarker for liver failure. Copyright © 2013 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Albumins - metabolism
- Biomarkers - metabolism
- Case-Control Studies -
- End Stage Liver Disease - metabolism End Stage Liver Disease - mortality End Stage Liver Disease - physiopathology
- Female -
- Humans -
- Kaplan-Meier Estimate -
- Liver - metabolism Liver - physiopathology
- Liver Cirrhosis - metabolism Liver Cirrhosis - mortality Liver Cirrhosis - physiopathology
- Male -
- Middle Aged -
- Oxidative Stress - physiology
- Protein Binding - physiology
- Sepsis - metabolism Sepsis - mortality Sepsis - physiopathology
- Serum Albumin - metabolism
- Survival Rate -
- Find related publications in this database (Keywords)
- Oxidative stress
- Cirrhosis
- Mercaptalbumin
- Non-mercaptalbumin
- Dansylsarcosine