Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Molcanyi, M; Bosche, B; Kraitsy, K; Patz, S; Zivcak, J; Riess, P; El Majdoub, F; Hescheler, J; Goldbrunner, R; Schäfer, U.
Pitfalls and fallacies interfering with correct identification of embryonic stem cells implanted into the brain after experimental traumatic injury.
J Neurosci Methods. 2013; 215(1):60-70
Doi: 10.1016/j.jneumeth.2013.02.012
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Molcanyi Marek
- Schäfer Ute
- Co-authors Med Uni Graz
- Kraitsy Klaus
- Patz Silke
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- Abstract:
- Cell-therapy was proposed to be a promising tool in case of death or impairment of specific cell types. Correct identification of implanted cells became crucial when evaluating the success of transplantation therapy. Various methods of cell labeling have been employed in previously published studies. The use of intrinsic signaling of green fluorescent protein (GFP) has led to a well known controversy in the field of cardiovascular research. We encountered similar methodological pitfalls after transplantation of GFP-transfected embryonic stem cells into rat brains following traumatic brain injury (TBI). As the identification of implanted graft by intrinsic autofluorescence failed, anti-GFP labeling coupled to fluorescent and conventional antibodies was needed to visualize the implanted cells. Furthermore, different cell types with strong intrinsic autofluorescence were found at the sites of injury and transplantation, thus mimicking the implanted stem cells. GFP-positive stem cells were correctly localized, using advanced histological techniques. The activation of microglia/macrophages, accompanying the transplantation post TBI, was shown to be a significant source of artefacts, interfering with correct identification of implanted stem cells. Dependent on the strategy of stem cell tracking, the phagocytosis of implanted cells as observed in this study, might also impede the interpretation of results. Critical appraisal of previously published data as well as a review of different histological techniques provide tools for a more accurate identification of transplanted stem cells. Copyright © 2013 Elsevier B.V. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Brain - cytology
- Brain Injuries - pathology
- Cell Fusion -
- Cell Line -
- Cells, Cultured -
- Embryonic Stem Cells - physiology
- Fluorescent Dyes -
- Immunohistochemistry -
- Magnetic Resonance Imaging -
- Male -
- Rats -
- Rats, Sprague-Dawley -
- Stem Cell Transplantation - methods
- Find related publications in this database (Keywords)
- Embryonic stem cell
- Cell transplantation
- GFP
- Traumatic brain injury
- Macrophage
- Phagocytosis
- In vivo trafficking
- Autofluorescence