Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Sturm, EM; Dyer, KD; Percopo, CM; Heinemann, A; Rosenberg, HF.
Chemotaxis of bone marrow derived eosinophils in vivo: a novel method to explore receptor-dependent trafficking in the mouse.
Eur J Immunol. 2013; 43(8):2217-2228
Doi: 10.1002/eji.201343371
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- Führende Autor*innen der Med Uni Graz
- Böhm Eva
- Co-Autor*innen der Med Uni Graz
- Heinemann Akos
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- Abstract:
- Here, we describe a novel method via which ex vivo cultured mouse bone marrow derived eosinophils (bmEos) can be adoptively transferred into recipient mice in order to study receptor-dependent recruitment to lung tissue in vivo. Intratracheal instillation of recombinant human eotaxin-2 (hCCL24) prior to introduction of bmEos via tail vein injection resulted in an approximately fourfold increase in Siglec F-positive/CD11c-negative eosinophils in the lungs of eosinophil-deficient ΔdblGATA recipient mice compared with controls. As anticipated, bmEos generated from CCR3-gene-deleted mice did not migrate to the lung in response to hCCL24 in this model, indicating specific receptor dependence. BmEos generated from GFP-positive BALB/c mice responded similarly to hCCL24 in vitro and were detected in lung tissue of BALB/c WT as well as BALB/c ΔdblGATA eosinophil-deficient recipient mice, at approximately fourfold (at 5 h post-injection) and approximately threefold (at 24 h postinjection) over baseline, respectively. Comparable results were obtained with GFP-positive C57BL/6 bmEos responding to intratracheal hCCL24 in C57BL/6 ΔdblGATA recipient mice. The use of ex vivo cultured bmEos via one or more of these methods offers the possibility of manipulating bmEos prior to transfer into a WT or gene-deleted recipient host. Thus, this chemotaxis model represents a novel and robust tool for pharmacological studies in vivo.
- Find related publications in this database (using NLM MeSH Indexing)
- Adoptive Transfer -
- Animals -
- Antigens, CD11c - biosynthesis
- Bone Marrow Cells - cytology
- Cells, Cultured -
- Chemokine CCL24 - pharmacology
- Chemotaxis, Leukocyte - immunology
- Eosinophils - cytology
- Green Fluorescent Proteins - genetics
- Lung - immunology
- Mice -
- Mice, Inbred BALB C -
- Mice, Inbred C57BL -
- Mice, Knockout -
- Receptors, CCR3 - genetics
- Find related publications in this database (Keywords)
- Bone morrow derived eosinophils
- CCL24
- Chemotaxis
- Immunology
- Mouse model