Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Seiringer, M; Maurer, M; Shebl, O; Dreier, K; Tews, G; Ziehr, S; Schappacher-Tilp, G; Petek, E; Ebner, T.
Efficacy of a sperm-selection chamber in terms of morphology, aneuploidy and DNA packaging.
Reprod Biomed Online. 2013; 27(1):81-88
Doi: 10.1016/j.rbmo.2013.03.013
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- Führende Autor*innen der Med Uni Graz
- Ebner Thomas
- Co-Autor*innen der Med Uni Graz
- Petek Erwin
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- Abstract:
- Since most current techniques analysing spermatozoa will inevitably exclude these gametes from further use, attempts have been made to enrich semen samples with physiological spermatozoa with good prognosis using special sperm-processing methods. A particular sperm-selection chamber, called the Zech-selector, was found to be effective in completely eliminating spermatozoa with DNA strand breaks. The aim of this study was to further analyse the subgroup of spermatozoa accumulated using the Zech-selector. In detail, the potential of the chamber to select for proper sperm morphology, DNA status and chromatin condensation was tested. Two samples, native and processed semen, of 53 patients were analysed for sperm morphology (×1000, ×6300), DNA packaging (fragmentation, chromatin condensation) and chromosomal status (X, Y, 18). Migration time (the time needed for proper sperm accumulation) was significantly correlated to fast progressive motility (P=0.002). The present sperm-processing method was highly successful with respect to all parameters analysed (P<0.001). In particular, spermatozoa showing numeric (17.4% of patients without aneuploidy) or structural chromosomal abnormalities (90% of patients without strand-breaks) were separated most effectively. To summarize, further evidence is provided that separating spermatozoa without exposure to centrifugation stress results in a population of highly physiological spermatozoa.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aneuploidy -
- Cell Separation - instrumentation
- DNA Fragmentation -
- DNA Packaging -
- Humans -
- Male -
- Middle Aged -
- Prospective Studies -
- Semen Analysis -
- Sperm Motility -
- Spermatozoa - cytology
- Find related publications in this database (Keywords)
- chromatin decondensation
- DNA strand break
- sperm aneuploidy
- sperm processing
- vacuole