Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Fogeron, ML; Muller, H; Schade, S; Dreher, F; Lehmann, V; Kuhnel, A; Scholz, AK; Kashofer, K; Zerck, A; Fauler, B; Lurz, R; Herwig, R; Zatloukal, K; Lehrach, H; Gobom, J; Nordhoff, E; Lange, BMH; .
LGALS3BP regulates centriole biogenesis and centrosome hypertrophy in cancer cells.
NAT COMMUN. 2013; 4(2): 1531-1531.
Doi: 10.1038/ncomms2517
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- Co-Autor*innen der Med Uni Graz
- Kashofer Karl
- Zatloukal Kurt
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- Abstract:
- Centrosome morphology and number are frequently deregulated in cancer cells. Here, to identify factors that are functionally relevant for centrosome abnormalities in cancer cells, we established a protein-interaction network around 23 centrosomal and cell-cycle regulatory proteins, selecting the interacting proteins that are deregulated in cancer for further studies. One of these components, LGALS3BP, is a centriole- and basal body-associated protein with a dual role, triggering centrosome hypertrophy when overexpressed and causing accumulation of centriolar substructures when downregulated. The cancer cell line SK-BR-3 that overexpresses LGALS3BP exhibits hypertrophic centrosomes, whereas in seminoma tissues with low expression of LGALS3BP, supernumerary centriole-like structures are present. Centrosome hypertrophy is reversed by depleting LGALS3BP in cells endogenously overexpressing this protein, supporting a direct role in centrosome aberration. We propose that LGALS3BP suppresses assembly of centriolar substructures, and when depleted, causes accumulation of centriolar complexes comprising CPAP, acetylated tubulin and centrin.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antigens, Neoplasm - genetics Antigens, Neoplasm - metabolism
- Carrier Proteins - genetics Carrier Proteins - metabolism
- Cell Line, Tumor -
- Centrioles - metabolism Centrioles - pathology Centrioles - ultrastructure
- Chromatography, Affinity -
- Extracellular Matrix Proteins - metabolism
- Gene Expression Regulation, Neoplastic -
- Gene Knockdown Techniques -
- Glycoproteins - genetics Glycoproteins - metabolism
- HEK293 Cells -
- Humans -
- Hypertrophy -
- Male -
- Microtubules - metabolism Microtubules - ultrastructure
- Mitotic Spindle Apparatus - metabolism Mitotic Spindle Apparatus - ultrastructure
- Neoplasms - genetics Neoplasms - metabolism Neoplasms - pathology
- Protein Interaction Maps -
- Protein Transport -
- Protein-Serine-Threonine Kinases - metabolism
- RNA, Small Interfering - metabolism
- Rats -
- Rats, Sprague-Dawley -
- Seminoma - genetics Seminoma - pathology
- Tumor Markers, Biological - genetics Tumor Markers, Biological - metabolism