Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Oberauner, L; Zachow, C; Lackner, S; Högenauer, C; Smolle, KH; Berg, G.
The ignored diversity: complex bacterial communities in intensive care units revealed by 16S pyrosequencing.
Sci Rep. 2013; 3(5-6):1413-1413
Doi: 10.1038/srep01413
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Hoegenauer Christoph
- Smolle Karl-Heinz
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Indoor microbial communities play an important role in everyday human health, especially in the intensive care units (ICUs) of hospitals. We used amplicon pyrosequencing to study the ICU microbiome and were able to detect diverse sequences, in comparison to the currently used standard cultivation technique that only detected 2.5% of the total bacterial diversity. The phylogenetic spectrum combined species associated with the outside environment, taxa closely related to potential human pathogens, and beneficials as well as included 7 phyla and 76 genera. In addition, Propionibacterium spp., Pseudomonas spp., and Burkholderia spp. were identified as important sources of infections. Despite significantly different bacterial area profiles for floors, medical devices, and workplaces, similarities by network analyses and strains with identical molecular fingerprints were detected. This information will allow for new assessment of public health risks in ICUs, help create new sanitation protocols, and further our understanding of the development of hospital-acquired infections.
- Find related publications in this database (using NLM MeSH Indexing)
- Bacteria - classification Bacteria - genetics
- Burkholderia - genetics Burkholderia - isolation & purification
- Environmental Microbiology -
- Equipment and Supplies - microbiology
- Humans -
- Intensive Care Units -
- Phylogeny -
- Principal Component Analysis -
- Propionibacterium - genetics Propionibacterium - isolation & purification
- Pseudomonas - genetics Pseudomonas - isolation & purification
- RNA, Ribosomal, 16S - genetics
- Sequence Analysis, DNA -