Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Mrakovcic, M; Absenger, M; Riedl, R; Smole, C; Roblegg, E; Fröhlich, LF; Fröhlich, E.
Assessment of long-term effects of nanoparticles in a microcarrier cell culture system.
PLoS One. 2013; 8(2):e56791-e56791
Doi: 10.1371/journal.pone.0056791
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- Führende Autor*innen der Med Uni Graz
- Fröhlich Eleonore
- Fröhlich Maria
- Co-Autor*innen der Med Uni Graz
- Absenger-Novak Markus
- Fröhlich Leopold F.
- Riedl Regina
- Smole Claudia
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- Abstract:
- Nano-sized materials could find multiple applications in medical diagnosis and therapy. One main concern is that engineered nanoparticles, similar to combustion-derived nanoparticles, may cause adverse effects on human health by accumulation of entire particles or their degradation products. Chronic cytotoxicity must therefore be evaluated. In order to perform chronic cytotoxicity testing of plain polystyrene nanoparticles on the endothelial cell line EAhy 926, we established a microcarrier cell culture system for anchorage-dependent cells (BioLevitator(TM)). Cells were cultured for four weeks and exposed to doses, which were not cytotoxic upon 24 hours of exposure. For comparison, these particles were also studied in regularly sub-cultured cells, a method that has traditionally been used to assess chronic cellular effects. Culturing on basal membrane coated microcarriers produced very high cell densities. Fluorescent particles were mainly localized in the lysosomes of the exposed cells. After four weeks of exposure, the number of cells exposed to 20 nm polystyrene particles decreased by 60% as compared to untreated controls. When tested in sub-cultured cells, the same particles decreased cell numbers to 80% of the untreated controls. Dose-dependent decreases in cell numbers were also noted after exposure of microcarrier cultured cells to 50 nm short multi-walled carbon nanotubes. Our findings support that necrosis, but not apoptosis, contributed to cell death of the exposed cells in the microcarrier culture system. In conclusion, the established microcarrier model appears to be more sensitive for the identification of cellular effects upon prolonged and repeated exposure to nanoparticles than traditional sub-culturing.
- Find related publications in this database (using NLM MeSH Indexing)
- Biological Transport -
- Cell Culture Techniques - methods
- Cell Death - drug effects
- Cell Line -
- Humans -
- Intracellular Space - drug effects Intracellular Space - metabolism
- Microtechnology - methods
- Nanoparticles - chemistry Nanoparticles - toxicity
- Poly(ADP-ribose) Polymerases - metabolism
- Polystyrenes - chemistry Polystyrenes - metabolism Polystyrenes - toxicity
- Time Factors -
- Toxicity Tests - methods