Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Dunker, S; Kleinert, R; Faulborn, J.
Immunohistologic studies of the vitreous humor
OPHTHALMOLOGE. 1998; 95(1): 8-12.
Doi: 10.1007/s003470050228
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Kleinert Reinhold
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- Abstract:
- BACKGROUND: Immunohistological staining of the vitreous is difficult because of its high water content. We present a method for immunohistological staining of celloidin-embedded eyes. Results from diabetic and non-diabetic eyes are demonstrated. METHOD: Diabetic and non-diabetic eyes were firstly immersed in formol and then slowly dehydrated using rising concentrations of glycerine (sink method). Subsequently, the whole globe was embedded in celloidin and cut into 200-micron sections. Control areas of interest were dissected from the 200 microns sections under a lightmicroscope. These specimens were then embedded in paraffin and cut into 7-micron sections. The 7-micron sections were immunohistochemically stained for type I-collagen, type IV-collagen, fibronectin and laminin. RESULTS: This method makes immunohistochemical staining of the vitreous possible. Type IV collagen, laminin and fibronection were found at higher concentrations in diabetic eyes than in normal eyes. Type I collagen was detected in neither diabetic nor in normal eyes. CONCLUSIONS: Our method of examination allows immunohistological staining of the vitreous in its place of origin. Although our method is time consuming, it has some advantages over biochemical analysis: Even minimal changes and their exact distribution can be detected. Our first results show that the vitreous is built up inhomogeneously and that pathological influence can cause structural changes.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Aged, 80 and over -
- Collagen - analysis
- Diabetes Mellitus, Type 2 - pathology
- Diabetic Retinopathy - pathology
- Female - pathology
- Fibronectins - analysis
- Humans - analysis
- Immunoenzyme Techniques - analysis
- Laminin - analysis
- Male - analysis
- Middle Aged - analysis
- Vitreous Body - pathology
- Find related publications in this database (Keywords)
- Laminin
- Fibronectin
- Collagen Type IV
- Vitreoretinal Interface
- Type II Diabetes