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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Bondarenko, AI; Jean-Quartier, C; Malli, R; Graier, WF.
Characterization of distinct single-channel properties of Ca²⁺ inward currents in mitochondria.
Pflugers Arch. 2013; 465(7):997-1010
Doi: 10.1007/s00424-013-1224-1
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- Leading authors Med Uni Graz
- Bondarenko Oleksandr
- Graier Wolfgang
- Co-authors Med Uni Graz
- Jean-Quartier Claire
- Malli Roland
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- Abstract:
- Previous studies have demonstrated several molecularly distinct players involved in mitochondrial Ca(2+) uptake. In the present study, electrophysiological recordings on mitoplasts that were isolated from HeLa cells were performed in order to biophysically and pharmacologically characterize Ca(2+) currents across the inner mitochondrial membrane. In mitoplast-attached configuration with 105 mM Ca(2+) as a charge carrier, three distinct channel conductances of 11, 23, and 80 pS were observed. All types of mitochondrial currents were voltage-dependent and essentially depended on the presence of Ca(2+) in the pipette. The 23 pS channel exhibited burst kinetics. Though all channels were sensitive to ruthenium red, their sensitivity was different. The 11 and 23 pS channels exhibited a lower sensitivity to ruthenium red than the 80 pS channel. The activities of all channels persisted in the presence of cylosporin A, CGP 37187, various K(+)-channel inhibitors, and Cl(-) channel blockers disodium 4,4'-diisothiocyanatostilbene-2,2'-disulfonate and niflumic acid. Collectively, our data identified multiple conductances of Ca(2+) currents in mitoplasts isolated from HeLa cells, thus challenging the dogma of only one unique mitochondrial Ca(2+) uniporter.
- Find related publications in this database (using NLM MeSH Indexing)
- Action Potentials -
- Calcium - metabolism
- Calcium Channels - classification
- Cyclosporine - pharmacology
- HeLa Cells -
- Humans -
- Kinetics -
- Mitochondrial Membranes - metabolism
- Potassium Channel Blockers - pharmacology
- Ruthenium Red - pharmacology
- Find related publications in this database (Keywords)
- Mitochondrial Ca2+ channels
- Mitoplast
- Mitochondria
- Ca2+ signaling
- Patch clamp