Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Merkel, OM; Marsh, LM; Garn, H; Kissel, T.
Flow cytometry-based cell type-specific assessment of target regulation by pulmonary siRNA delivery.
Methods Mol Biol. 2013; 948(3):263-273
Doi: 10.1007/978-1-62703-140-0_18
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- Co-Autor*innen der Med Uni Graz
- Marsh Leigh
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- Abstract:
- Pulmonary siRNA delivery has attracted strong interest and has been reported to successfully mediate target gene knockdown in a number of disease models. However, the nature of the epithelial cells that eventually take up siRNA and the question if other lung cell types may also be transfected have so far been neglected. Therefore, we describe here a flow cytometry-based method using transgenic enhanced green fluorescence protein-expressing mice (EGFP mice) for the differentiation of transfected lung cell populations based on their antigen expression.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Base Sequence -
- Bronchoalveolar Lavage -
- Female -
- Flow Cytometry - methods
- Gene Knockdown Techniques -
- Green Fluorescent Proteins - deficiency Green Fluorescent Proteins - genetics
- Humans -
- Lung - cytology Lung - metabolism
- Male -
- Mice -
- RNA, Small Interfering - genetics RNA, Small Interfering - metabolism
- Spectrometry, Fluorescence -
- Transfection - methods