Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Gewählte Publikation:

Hinterleitner, TA; Saada, JI; Berschneider, HM; Powell, DW; Valentich, JD.
IL-1 Stimulates Intestinal Myofibroblast Cox Gene-Expression and Augments Activation of Cl- Secretion in T84 Cells
AMER J PHYSIOL, CELL PHYSIOL. 1996; 40: C1262-C1268. Doi: 10.1152/ajpcell.1996.271.4.C1262
Web of Science FullText FullText_MUG

Führende Autor*innen der Med Uni Graz: Hinterleitner Thomas
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Abstract:: Because interleukin-l (IL-1) is an important mediator in the inflamed intestine, its effects on enterocyte-subepithelial myofibroblast (SEMF) interaction were investigated in vitro. Acutely juxtaposing T84 cells with 18Co or P2JF SEMF preincubated with IL-1 alpha significantly enhanced T84 short-circuit current (I-sc) responsiveness to secretagogues in com parison to SEMF not activated by IL-1 alpha. The sensitivity of T84 cell I-sc to Ca2+-dependent, but not adenosine 3',5'-cyclic monophosphate-dependent, secretagogues was augmented by IL-1 alpha-treated SEMF. These effects of IL-1 alpha are directly correlated with SEMF prostaglandin E(2) (PGE(2)) production. Both IL-1 alpha augmentation of Cl- secretagogue responsiveness and PGE(2) formation were inhibited by IL-1 receptor antagonist. Within 5 h, IL-1 alpha stimulated a 10-fold increase in cyclooxygenase (COX)-2 steady-state mRNA levels in 18Co cells. In contrast, COX-1 message levels increased more slowly to two- to threefold above control levels after 24 h incubation. These results demonstrate that the proinflammatory cytokine IL-1 alpha accentuates intestinal SEMF augmentation of enterocyte responsiveness to Ca2+-dependent Cl- secretagogues. PGE(2) is an important mediator of SEMF-enterocyte interaction. The effects of IL-1 alpha on SEMF PGE(2) productions are, at least in part, due to stimulation of COX gene expression.
Find related publications in this database (Keywords): Interleukin-1; Cyclooxygenase; Prostaglandins; Intestines