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SHR Neuro Cancer Cardio Lipid Metab Microb
Fiegel, HC; Pryymachuk, G; Rath, S; Bleiziffer, O; Beier, JP; Bruns, H; Kluth, D; Metzger, R; Horch, RE; Till, H; Kneser, U; .
Foetal hepatocyte transplantation in a vascularized AV-Loop transplantation model in the rat.
J Cell Mol Med. 2010; 14(1-2):267-274
Doi: 10.1111/j.1582-4934.2008.00369.x
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- Co-authors Med Uni Graz
- Till Holger
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- Abstract:
- The use of foetal liver cells (FLC) in the context of hepatic tissue engineering might permit efficient in vitro expansion and cryopreservation in a cell bank. A prerequisite for successful application of bioartificial liver tissue is sufficient initial vascularization. In this study, we evaluated the transplantation of fibrin gel-immobilized FLC in a vascularized arterio-veno-venous (AV)-loop model. FLC were isolated from embryonic/foetal (ED 16) rat livers and were enriched by using magnetic cell sorting (MACS). After cryopreservation, FLC were labelled by pkh-26. Cells were transplanted in a fibrin matrix into a subcutaneous chamber containing a microsurgically created AV-loop in the femoral region of the recipient rat. The chambers were explanted after 14 days. Subcutaneous implants without an AV-loop and cell-free implants served as controls. Fluorescence microscopy of the constructs was used to identify pkh-26(+)- donor cells. Characterization was performed by RT-PCR and immunhistology (IH) for CK-18 and CD31. Transplantation of FLC using the AV-loop permitted a neo-tissue formation in the fibrin matrix. A high-density vascularization was observed in the AV-loop constructs as shown by CD31 IH. Viable foetal donor cells were detected which expressed CK-18. FLC can be successfully used for heterotopic transplantation. Fibrin matrix permits rapid blood vessel ingrowth from the AV-loop and supports engraftment of FLC. It is therefore an appropriate environment for hepatocyte transplantation in combination with microsurgical vascularization strategies. Transplantation of fibrin gel-immobilized FLC may be a promising approach for the development of highly vascularized in vivo tissue-engineering-based liver support systems.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Culture Techniques -
- Cell Differentiation -
- Female -
- Fetus - cytology
- Fibrin - metabolism
- Hepatocytes - cytology Hepatocytes - physiology Hepatocytes - transplantation
- Humans -
- Immunomagnetic Separation -
- Liver, Artificial -
- Pregnancy -
- Rats -
- Rats, Inbred Lew -
- Tissue Engineering - methods
- Tissue Scaffolds - chemistry
- Find related publications in this database (Keywords)
- foetal transplantation
- neo-vascularization
- liver cell transplantation
- hepatic tissue engineering
- AV loop