Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Muensterer, OJ; Flemmer, AW; Bergmann, F; Hajek, KS; Lu, HQ; Simbruner, G; Deprest, JA; Till, H.
Postnatal lung mechanics, lung composition, and surfactant synthesis after tracheal occlusion vs prenatal intrapulmonary instillation of perfluorocarbon in fetal rabbits
J PEDIATR SURG. 2005; 40(1): 26-31.
Doi: 10.1016/j.jpedsurg.2004.09.008
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Till Holger
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- Abstract:
- Background/Purpose: Fetal tracheal occlusion (TO) accelerates lung growth but decreases surfactant production. We have previously shown that instillation of perfluorooctylbromide (PFOB) into fetal rabbit lungs leads to lung growth similar to TO. This study compares neonatal lung mechanics and surfactant production after prenatal intrapulmonary PFOB instillation vs TO. Methods: In each of 18 pregnant rabbits on gestational day 27, sets of 4 fetuses underwent either (1) intrapulmonary instillation of 1 mL PFOB, (2) TO, (3) instillation of 1 mL 0.9% NaCl (saline), and (4) hysteroamniotomy without fetal manipulation (control). Fetuses were born by cesarean delivery after 48 hours. Fetuses of 12 rabbits were mechanically ventilated for 15 minutes to evaluate lung compliance and airway resistance. Pulmonary surfactant protein B (SP-B) was quantified by immunohistochemistry in fetuses of the remaining 6 rabbits. Results: Compliance was decreased in the TO group after cesarean delivery (0.33 +/- 0.13 mL/cm H2O) compared with PFOB (0.59 +/- 0.12 mL/cm H2O), saline (0.50 +/- 0.12 mL/cm H2O), and control (0.52 +/- 0.10 mL/cm H2O) fetuses. Mean fetal lung to body weight ratio was higher in TO and PFOB fetuses compared with saline and control. Higher water content and lower numbers of surfactant protein B-positive cells were found in the TO-treated fetuses. Conclusions: Both prenatal intrapulmonary instillation of PFOB and TO accelerate lung growth, but TO is associated with decreased postnatal lung compliance, possibly influenced by decreased surfactant production and increased fluid retention. Conversely, instillation of PFOB preserved lung compliance and surfactant synthesis. (C) 2005 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Administration, Inhalation -
- Airway Resistance - drug effects
- Animals -
- Body Composition -
- Female -
- Fetal Organ Maturity - drug effects
- Fluorocarbons - administration and dosage
- Ligation -
- Lung - drug effects Lung - embryology
- Lung Compliance - drug effects
- Models, Animal -
- Organ Size - drug effects
- Pregnancy -
- Pulmonary Surfactant-Associated Protein B - biosynthesis
- Rabbits -
- Respiration, Artificial -
- Respiratory Mechanics - drug effects
- Respiratory System Agents - administration and dosage
- Trachea - surgery
- Find related publications in this database (Keywords)
- per-fluorocarbon
- tracheal occlusion
- lung mechanics
- compliance
- resistance
- fetal rabbit