Medizinische Universität Graz - Research portal
Selected Publication:
Till, H; Metzger, R; Mempel, T; Boehm, R; Joppich, I.
Proliferation, zonal maturation, and steroid production of fetal adrenal transplants in adrenalectomized rats
Pediatr Surg Int. 2000; 16(4):293-296
Doi: 10.1007/s003830050747
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Till Holger
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- The present study investigated the histologic maturation, proliferative capacity, and steroid production of fetal adrenal transplants (Tx) in adrenalectomized rats. A pair of fetal adrenal glands (18-20 days of gestation) was transplanted into the omentum of syngeneic Lewis rats (n = 45). Four weeks later, in 5 animals the grafts were excised for morphologic evaluation. Proliferation was investigated by immunohistochemical staining for KI-67 protein and quantified by the proliferation index (PI = positive cells/100 counts). All other hosts (Tx; n = 40) underwent bilateral adrenalectomy (AE) to induce Addisonian crisis. Postoperatively, survival and concentrations of potassium, sodium, aldosterone, and corticosterone were recorded for 6 months. These data were compared to controls (C=only AE; n = 30) and a sham group (S; n = 10). At the end of the study period all surviving hosts were killed for histologic examination of grafts. At 4 weeks post-Tx the adrenal grafts demonstrated a distinct zona glomerulosa and frequent proliferation with a PI of 0.084, comparable to normal control (0.092). Following AE survival was significantly prolonged in Tx (86% vs 12% of C, P < 0.05). Control animals developed severe hyponatremia and hyperkalemia, whereas in Tx only transient signs of Addisonian crisis were recorded. Levels of aldosterone dropped within 7 days in the Tx and C groups, but returned to normal for Tx within 8 weeks. Corticosterone levels of Tx animals fell to 25% within week, but steadily increased to 70% by the end of the study. At 6 months, grafts revealed a mature adrenocortical structure with little proliferative activity, which was comparable to controls. In a syngeneic rat model fetal adrenal transplants thus mature and proliferate to provide sufficient steroid production for adrenalectomized hosts.
- Find related publications in this database (using NLM MeSH Indexing)
- Adrenal Glands - embryology Adrenal Glands - pathology Adrenal Glands - transplantation
- Adrenalectomy -
- Animals -
- Cell Division -
- Corticosterone - analysis
- Fetus - surgery
- Immunohistochemistry -
- Models, Animal -
- Rats -
- Rats, Inbred Lew -
- Transplantation, Isogeneic -
- Find related publications in this database (Keywords)
- fetal transplantation
- proliferation
- adrenal glands
- Addisonian crisis
- rat