Medizinische Universität Graz - Research portal
Selected Publication:
Behrends, U; Jandl, T; Golbeck, A; Lechner, B; Muller-Weihrich, S; Schmid, I; Till, H; Berthold, F; Voltz, R; Mautner, JM.
Novel products of the HUD, HUC, NNP-1 and alpha-internexin genes identified by autologous antibody screening of a pediatric neuroblastoma library
Int J Cancer. 2002; 100(6):669-677
Doi: 10.1002/ijc.10550
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Till Holger
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- Abstract:
- Autologous serological screening of a cDNA expression library (SEREX) derived from childhood neuroblastoma led to the identification of 10 different antigens, including 6 novel gene products. The novel antigen 018INX was derived from a small open reading frame in a region of alpha-internexin mRNA that was previously described as 3' untranslated region. 018INX thus represents a novel type of tumor antigen. Five novel gene products were derived from NNP-1 (NNP3) and Hu genes (HuC-L, HuD3, HuDY, HuD1pro(c)). As indicated by sequence analysis, these antigens were generated by alternative splicing and/or alternative promoter usage or allelic polymorphism. mRNA expression analyses revealed different tissue restrictions of novel compared to known HuD and NNP-1 transcripts in normal and malignant tissues. The expressions patterns of distinct transcripts indicated potential clinical meanings as diagnostic and/or prognostic tissue markers. When kinetics of serum antibody titres against SEREX-defined antigens were compared to tumor load over time in our patient with neuroblastoma, we found 100-fold increases of anti-Hu and anti-018INX antibody titres preceding the clinical diagnosis of recurrent tumor growth after 2 years. When sera of pediatric patients with cancer (30) and healthy controls (30) were tested for humoral responses to SEREX-defined neuroblastoma antigens, we detected antibodies against all known antigens and NNP3 with low frequencies and titres in control sera, while anti-018INX and anti-Hu antibodies were found in cancer patients only. Our findings indicate that SEREX-defined tumor antigens might provide novel tools for understanding and treatment of this aggressive childhood malignancy.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Amino Acid Sequence -
- Base Sequence -
- Blotting, Southern -
- Carrier Proteins - genetics
- Case-Control Studies -
- Child, Preschool -
- DNA, Complementary - metabolism
- Female -
- Gene Library -
- Hu Paraneoplastic Encephalomyelitis Antigens -
- Humans -
- Intermediate Filament Proteins -
- Models, Genetic -
- Molecular Sequence Data -
- Nerve Tissue Proteins - genetics
- Neuroblastoma - genetics
- Nuclear Proteins - genetics
- RNA, Messenger - metabolism
- RNA-Binding Proteins - genetics
- Reverse Transcriptase Polymerase Chain Reaction -
- Sequence Homology, Amino Acid -
- Time Factors -
- Find related publications in this database (Keywords)
- SEREX
- neuroblastoma
- tumor antigen
- tumor marker
- Hu syndrome