Medizinische Universität Graz - Research portal
Selected Publication:
Metzger, R; Mempel, T; Joppich, I; Till, H.
Organ-specific distribution of major histocompatibility antigens in rats
Pediatr Surg Int. 2000; 16(4):285-292
Doi: 10.1007/s003830050746
Web of Science
PubMed
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FullText_MUG
- Co-authors Med Uni Graz
- Till Holger
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- Abstract:
- The present study systematically investigated the expression and distribution of the major histocompatibility complex (MHC) classes I and II in the rat. About 150 native tissue probes from eight adult Lewis rats were taken, representative for most organs, tissues, and the vascular system. MHC expression was analyzed by two monoclonal antibodies (mAb) generated against the non-polymorphic determinants of rat MHC class I (Ox-18) and class II (Ox-6). Immunoreactivities were compared to those of different endothelial (HIS52, TLD-3A12, Ox-43, REHA-1 antigen), histiocytic (ED1, ED2), B-cell (RLN-9D3), and T-cell (MRC Ox-52) markers. A nonspecific mAb (MR12/53) served as a negative control. Pretested concentrations on various tissues and the alkaline phosphatase-anti-alkaline phosphatase technique allowed semiquantitative evaluation of serial cryostat tissue sections. MHC class I expression was detected on most immunocompetent cells. Endothelial cells were stained heterogeneously along the vascular system and the organ-specific microcirculation. Furthermore, some organs showed staining of parenchymal cells. MHC class II was found on all immunocompetent cells positive for the B-cell marker and about 15% of cells positive for the histiocytic markers. Besides the well-known expression of MHC class II in the outer zone of the renal proximal tubule, further organ-specific cell forms were found positive. In conclusion, the present study outlines tissue-specific distribution of MHC I/ II and implies that each organ carries a variable immunologic burden that needs to be considered for any transplantation model.
- Find related publications in this database (using NLM MeSH Indexing)
- Adrenal Cortex - metabolism Adrenal Cortex - pathology
- Adrenal Medulla - metabolism Adrenal Medulla - pathology
- Animals -
- Antibodies, Monoclonal -
- Histocompatibility Antigens Class I - metabolism
- Histocompatibility Antigens Class II - metabolism
- Immunoenzyme Techniques -
- Islets of Langerhans - metabolism Islets of Langerhans - pathology
- Male -
- Rats -
- Rats, Inbred Lew -
- Thyroid Gland - metabolism Thyroid Gland - pathology
- Tissue Distribution -
- Find related publications in this database (Keywords)
- major histocompatibility complex (MHC)
- rat
- immunohistochemistry
- distribution