Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Fiegel, HC; Kaifi, JT; Wachowiak, R; Quaas, A; Aridome, K; Ichihara-Tanaka, K; Muramatsu, T; Metzger, R; Izbicki, JR; Erttmann, R; Kluth, D; Till, H.
Midkine is highly expressed in neuroblastoma tissues
Pediatr Surg Int. 2008; 24(12):1355-1359
Doi: 10.1007/s00383-008-2263-0
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Till Holger
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- Abstract:
- Purpose Neuroblastoma (NBL) is a tumor from neural crest cells, and is the most frequent solid tumor in children. Midkine (MK) is a pleiotropin analogon, which is frequently expressed in neuronal and epithelial tumors and is a marker for a poor clinical outcome. The aims of this study were to assess MK expression in NBL and investigate the correlation with clinical outcome. Methods Fifty-six specimens of NBL were stained for MK on a tissue microarray by immunohistochemistry (IHC). Fresh frozen tumor tissues were used for RNA isolation, and RT-PCR analysis for MK-mRNA expression was performed. Survival data, risk factors and disease stages were correlated with MK status assessed by IHC and RT-PCR analysis. Results MK-mRNA expression was found in the majority of the tumor tissues (75%), whereas MK protein could be detected only in 46% of the NBL by IHC. No correlation of MK status with survival, risk factors or disease stage was observed. Conclusion A majority of NBL express MK-mRNA, whereas not all MK mRNA positive tumors showed also a positive MK IHC staining. The high expression of MK-mRNA expression might present a promising target for new adenovirus-based gene therapeutic approaches for the treatment of NBL.
- Find related publications in this database (using NLM MeSH Indexing)
- Child, Preschool -
- Humans -
- Immunohistochemistry -
- Infant -
- Nerve Growth Factors - biosynthesis
- Neuroblastoma - metabolism
- Reverse Transcriptase Polymerase Chain Reaction -
- Tissue Array Analysis -
- Tumor Markers, Biological - biosynthesis
- Find related publications in this database (Keywords)
- Neuroblastoma (NBL)
- Midkine (MK)
- Tumor marker
- Childhood cancer