Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Puppa, G; Senore, C; Sheahan, K; Vieth, M; Lugli, A; Zlobec, I; Pecori, S; Wang, LM; Langner, C; Mitomi, H; Nakamura, T; Watanabe, M; Ueno, H; Chasle, J; Conley, SA; Herlin, P; Lauwers, GY; Risio, M.
Diagnostic reproducibility of tumour budding in colorectal cancer: a multicentre, multinational study using virtual microscopy.
Histopathology. 2012; 61(4):562-575
Doi: 10.1111/j.1365-2559.2012.04270.x
Web of Science
PubMed
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- Langner Cord
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- Abstract:
- Despite the established prognostic relevance of tumour budding in colorectal cancer, the reproducibility of the methods reported for its assessment has not yet been determined, limiting its use and reporting in routine pathology practice. A morphometric system within telepathology was devised to evaluate the reproducibility of the various methods published for the assessment of tumour budding in colorectal cancer. Five methods were selected to evaluate the diagnostic reproducibility among 10 investigators, using haematoxylin and eosin (H&E) and AE1-3 cytokeratin-immunostained, whole-slide digital scans from 50 pT1-pT4 colorectal cancers. The overall interobserver agreement was fair for all methods, and increased to moderate for pT1 cancers. The intraobserver agreement was also fair for all methods and moderate for pT1 cancers. Agreement was dependent on the participants' experience with tumour budding reporting and performance time. Cytokeratin immunohistochemistry detected a higher percentage of tumour budding-positive cases with all methods compared to H&E-stained slides, but did not influence agreement levels. An overall fair level of diagnostic agreement for tumour budding in colorectal cancer was demonstrated, which was significantly higher in early cancer and among experienced gastrointestinal pathologists. Cytokeratin immunostaining facilitated detection of budding cancer cells, but did not result in improved interobserver agreement. © 2012 Blackwell Publishing Ltd.
- Find related publications in this database (using NLM MeSH Indexing)
- Colorectal Neoplasms - pathology
- Humans -
- Microscopy - methods
- Observer Variation -
- Reproducibility of Results -
- Telepathology - methods
- Find related publications in this database (Keywords)
- colorectal cancer
- reproducibility
- tumour budding
- virtual microscopy