Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schittmayer, M; Glieder, A; Uhl, MK; Winkler, A; Zach, S; Schrittwieser, JH; Kroutil, W; Macheroux, P; Gruber, K; Kambourakis, S; Rozzell, JD; Winkler, M.
Old Yellow Enzyme-Catalyzed Dehydrogenation of Saturated Ketones
ADV SYNTH CATAL. 2011; 353(2-3): 268-274.
Doi: 10.1002/adsc.201000862
Web of Science
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- Führende Autor*innen der Med Uni Graz
- Schittmayer-Schantl Matthias
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- Abstract:
- Enzymes from extremophiles have always been of great interest for biotechnology because of their ruggedness against various stress factors. We have isolated, cloned, heterologously expressed and characterized a thermostable old yellow enzyme (OYE) from Geobacillus kaustophilus. In addition to the expected 'enone' reduction, GkOYE also catalyzes the reverse reaction, i.e., the desaturation of C-C bonds adjacent to a carbonyl to give the corresponding alpha,beta-unsaturated ketone. The reaction proceeds at the expense of molecular oxygen without the need for a nicotinamide cofactor and represents an environmentally benign alternative to known chemical dehydrogenation methods.
- Find related publications in this database (Keywords)
- dehydrogenation
- desaturation
- enzymatic oxidation
- old yellow enzyme
- oxidoreductases
- thermophiles