Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Steinleitner, K; Rampetsreiter, P; Köffel, R; Ramanathan, G; Mannhalter, C; Strobl, H; Wieser, R.
EVI1 and MDS1/EVI1 Expression During Primary Human Hematopoietic Progenitor Cell Differentiation into Various Myeloid Lineages.
Anticancer Res. 2012; 32(11):4883-4889
[OPEN ACCESS]
Web of Science
PubMed
- Co-Autor*innen der Med Uni Graz
- Köffel René
- Strobl Herbert
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Abstract:
- Background and Aim: Overexpression of ecotropic viral integration site 1 (EVI1) is associated with aggressive disease in myeloid leukemia. We therefore studied its expression and function in cluster of differentiation 34-positive (CD34(+)) primary human hetnatopoietic progenitor cells. Materials and Methods: CD34(+) cells were differentiated into various myeloid lineages using the appropriate cytokines. EVI1 expression was measured by quantitative real time reverse transcriptase-polymerase chain reaction (qRT-PCR) and intranuclear fluorescence-activated cell sorting (FACS). Experimental manipulation of EVI1 levels was achieved using retroviral infection. Results: EVI1 mRNA and its variant myelodysplastic syndrome 1 (MDS1)/EVI1, which gives rise to a partially antagonistic protein, were detectable in CD34(+) cells, but their levels declined rapidly during differentiation into the granulocyte, monocyte, dendritic, erythroid, and megakaryocyte lineages. Similarly, EVI1 protein levels decreased during myeloid differentiation. Attempts to experimentally express EVI1 in CD34(+) and U937 cells indicated that ectopic expression of EVI1 may cause growth arrest, apoptosis and/or senescence of human hematopoietic cells. Conclusion: EVI1 is expressed in human hematopoietic progenitor cells, but is down-regulated during differentiation. Ectopic expression of EVI1
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD34 - biosynthesis
- Cell Differentiation - physiology
- Cell Lineage - physiology
- DNA-Binding Proteins - biosynthesis
- Flow Cytometry -
- Hematopoietic Stem Cells - cytology
- Humans -
- Proto-Oncogenes -
- Real-Time Polymerase Chain Reaction -
- Reverse Transcriptase Polymerase Chain Reaction -
- Transcription Factors - biosynthesis
- U937 Cells -
- Find related publications in this database (Keywords)
- EVI1
- leukemia
- primary human hematopoietic cells
- myeloid differentiation