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SHR Neuro Cancer Cardio Lipid Metab Microb

Matthaei, M; Lackner, EM; Meng, H; Hicks, JL; Meeker, AK; Eberhart, CG; Jun, AS.
Tissue Microarray Analysis of Cyclin-Dependent Kinase Inhibitors p21 and p16 in Fuchs Dystrophy.
Cornea. 2013; 32(4):473-478 Doi: 10.1097/ICO.0b013e31826f324e [OPEN ACCESS]
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Co-authors Med Uni Graz: Lackner Eva-Maria
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Abstract:: Purpose: To investigate the novel application of tissue microarray (TMA) technology to corneal disease and to report altered protein expression of senescence-associated cyclin-dependent kinase inhibitors p21 and p16 in Fuchs endothelial corneal dystrophy (FECD). Methods: A TMA including 208 cores was generated from paraffin-embedded tissues, including corneal buttons of 50 FECD and 5 keratoconus patients retrieved after penetrating keratoplasty, 10 autopsy globes with nonpathologic corneas, and nonocular control specimens. TMA sections were immunolabeled for p21 and p16 and analyzed using a 9-grade scoring system (0-8). Result validation was performed by immunolabeling of individual whole tissue sections. Corneal endothelial p21 and p16 expression levels in FECD specimens compared with controls served as main outcome measures. Results: TMA immunohistochemical analysis disclosed increased endothelial expression levels of nuclear p21 in FECD specimens (P < 0.05) and an altered endothelial p16 expression pattern. Immunolabeling of whole tissue sections showed statistically significant endothelial overexpression of both proteins (p21 and p16, P < 0.05). Conclusions: The present study introduces TMA technology as a valuable tool for molecular high-throughput profiling of corneal tissues. It demonstrates p21 and p16 overexpression in the corneal endothelium of genetically undifferentiated FECD patients supporting a role of cellular senescence in the pathogenesis of FECD.
Find related publications in this database (using NLM MeSH Indexing): Aged -; Cyclin-Dependent Kinase Inhibitor p16 - metabolism; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Female -; Fuchs' Endothelial Dystrophy - metabolism; Humans -; Immunohistochemistry -; Male -; Middle Aged -; Tissue Array Analysis -
Find related publications in this database (Keywords): Fuchs dystrophy; tissue microarray; cyclin-dependent kinase inhibitor