Medizinische Universität Graz - Research portal
Selected Publication:
Prassl, R; Schuster, B; Laggner, P; Flamant, C; Nigon, F; Chapman, MJ.
Thermal stability of apolipoprotein B100 in low-density lipoprotein is disrupted at early stages of oxidation while neutral lipid core organization is conserved
Biochemistry. 1998; 37(3):938-944
Doi: 10.1021/bi971853f
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- Leading authors Med Uni Graz
- Prassl Ruth
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- Abstract:
- The time course of the unfolding characteristics of the protein moiety and of the thermotropic behavior of the core-located apolar lipids of highly homogeneous low-density lipoprotein (LDL) subspecies (d 1.030-1.040 g/mL) have been evaluated during transition metal- and azo radical-induced oxidation using differential scanning calorimetry. Apolipoprotein B100 (apo-B100) structure was highly sensitive to oxidative modification; indeed, a significant loss of thermal stability was observed at initial stages irrespective of whether oxidation was mediated by site-specific binding of copper ions or by free radicals generated during decomposition of azo compounds. Subsequently, thermal protein integrity was destroyed, as a result of potentially irreversible protein unfolding, cross-linking reactions, and aggregation. Our results suggest that even minimal oxidative modification of apo-B100 has a major impact on the stability of this large monomeric protein. By contrast, the core lipids, which consist primarily of cholesteryl esters and triglycerides and play a determinant role in the thermal transition occurring near physiological temperature, preserved features of an ordered arrangement even during propagation of lipid peroxidation.
- Find related publications in this database (using NLM MeSH Indexing)
- Amidines - pharmacology
- Apolipoprotein B-100 -
- Apolipoproteins B - chemistry Apolipoproteins B - metabolism
- Calorimetry, Differential Scanning -
- Copper - pharmacology
- Hot Temperature -
- Humans -
- Lipid Metabolism -
- Lipid Peroxidation - drug effects
- Lipids - chemistry
- Lipoproteins, LDL - chemistry Lipoproteins, LDL - drug effects Lipoproteins, LDL - metabolism