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Selected Publication:
Prassl, R; Chapman, JM; Nigon, F; Sara, M; Eschenburg, S; Betzel, C; Saxena, A; Laggner, P.
Crystallization and preliminary x-ray analysis of a low density lipoprotein from human plasma
J Biol Chem. 1996; 271(46):28731-28733
Doi: 10.1074/jbc.271.46.28731
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- Prassl Ruth
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- Abstract:
- Single crystals of human plasma low density lipoprotein (LDL), the major transport vehicle for cholesterol in blood, have been produced with a view to analysis of the three-dimensional structure by x-ray crystallography. Crystals with dimensions of approximately 200 x 100 x 50 microm have been reproducibly obtained from highly homogeneous LDL particle subspecies, isolated in the density ranges d = 1.0271-1. 0297 g/ml and d = 1.0297-1.0327 g/ml. Electron microscopic imaging of ultrathin-sectioned preparations of the crystals confirmed the existence of a regular, quasihexagonal arrangement of spherical particles of approximately 18 nm in diameter, thereby resembling the dimensions characteristic of LDL after dehydration and fixation. X-ray diffraction with synchrotron radiation under cryogenic conditions revealed the presence of well resolved diffraction spots, to a resolution of about 29 A. The diffraction patterns are indexed in terms of a triclinic lattice with unit cell dimensions of a = 16. 1 nm, b = 39.0 nm, c = 43.9 nm; alpha = 96.2 degrees, beta = 92.1 degrees, gamma = 102 degrees, and with space group P1.
- Find related publications in this database (using NLM MeSH Indexing)
- Crystallography, X-Ray -
- Humans -
- Lipoproteins, LDL - blood Lipoproteins, LDL - chemistry
- Microscopy, Electron -
- Protein Conformation -