Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Kostner, GM; Oettl, K; Jauhiainen, M; Ehnholm, C; Esterbauer, H; Dieplinger, H.
Human plasma phospholipid transfer protein accelerates exchange/transfer of alpha-tocopherol between lipoproteins and cells.
Biochem J. 1995; 305 ( Pt 2)(5):659-667
Doi: 10.1042/bj3050659
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Kostner Gerhard
- Co-Autor*innen der Med Uni Graz
- Öttl Karl
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- alpha-Tocopherol (alpha-T), an important anti-oxidant of plasma lipoproteins and cell membranes, is secreted from liver together with very-low-density lipoproteins into the blood stream. Other serum lipoprotein classes gain alpha-T by exchange and transfer processes. We show here that the lipoprotein-free d > 1.22 g/ml fraction of human or pig serum increases the exchange rate of alpha-T by a factor of 2-4 as compared with spontaneous exchange/transfer. The alpha-T exchange/transfer (alpha-TET) activity was purified by multiple-step column chromatography. It gave a single band in PAGE with an apparent molecular mass of 75 kDa, and was found to be identical with the phospholipid transfer protein (PLTP). PLTP catalysed alpha-T exchange between different lipoprotein classes, as well as the transfer of alpha-T from artificial liposomes to high-density lipoproteins. The alpha-TET activity measured with a newly developed assay in ten healthy people was 2.45 +/- 0.88 nmol.ml-1.h-1.alpha-TET activity was negatively correlated with plasma low-density lipoprotein-cholesterol (r = -0.75; P < 0.01). It is concluded that human PLTP catalyses exchange/transfer processes of alpha-T between lipid compartments. This factor may be of relevance in atherogenesis and tumour initiation and growth.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Biological Transport - drug effects
- Blood Proteins - metabolism
- Carrier Proteins - isolation and purification
- Cholesterol Ester Transfer Proteins - isolation and purification
- Erythrocyte Membrane - metabolism
- Fasting - metabolism
- Female - metabolism
- Glycoproteins - metabolism
- Humans - metabolism
- Lipoproteins - metabolism
- Lipoproteins, HDL - metabolism
- Lipoproteins, LDL - metabolism
- Lipoproteins, VLDL - metabolism
- Liposomes - metabolism
- Male - metabolism
- Membrane Proteins - isolation and purification
- Phospholipid Transfer Proteins - isolation and purification
- Sulfhydryl Reagents - pharmacology
- Swine - pharmacology
- Vitamin E - metabolism