Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Singer, G; Stokes, KY; Neil Granger, D.
Reactive oxygen and nitrogen species in sepsis-induced hepatic microvascular dysfunction.
Inflamm Res. 2013; 62(2):155-164
Doi: 10.1007/s00011-012-0562-3
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- Führende Autor*innen der Med Uni Graz
- Singer Georg
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- Abstract:
- Hepatic microvascular dysfunction is a critical event in the development of liver failure during sepsis. Activated blood cells and reactive oxygen and nitrogen species (RONS) have been implicated in the pathogenesis of sepsis. Intravital-videomicroscopy was used to determine whether RONS contribute to the recruitment of leukocytes/platelets in the hepatic microvasculature during sepsis. Six hours following cecal-ligation and puncture (CLP), disturbances of the hepatic microvasculature were assessed in WT-mice (C57Bl/6 J; n = 8), in mice lacking gp91(phox)(n = 5), overexpressing superoxide-dismutase (SOD, n = 8), in WT-mice treated with a NOS-inhibitor (l-NAME, n = 5), lacking nNOS, eNOS or iNOS (n = 5 each), treated with the NO-donor DetaNO (n = 5), in WT-mice treated with gadolinium-chloride (GdCl2, n = 5) and compared to a group of WT-mice following a sham operation (n = 8). Six hours post-CLP, the adhesion of leukocytes and platelets in terminal hepatic venules (THV) and sinusoids was quantified. In WT-mice, CLP elicited increases in the number of adherent leukocytes and platelets. Similar responses to CLP were noted in mice overexpressing SOD or lacking either eNOS or gp91(phox). The blood-cell recruitment was significantly blunted in septic iNOS-knockout mice and this response was reversed by pre-treatment with DetaNO. These findings suggest that iNOS-derived NO is a determinant of the pro-inflammatory phenotype assumed by the hepatic microvasculature during sepsis.
- Find related publications in this database (using NLM MeSH Indexing)
- Alanine Transaminase - blood
- Animals -
- Blood Pressure -
- Cell Adhesion -
- Cell Count -
- Cytokines - blood
- Gadolinium - pharmacology
- Leukocyte Count -
- Liver Diseases - etiology Liver Diseases - immunology Liver Diseases - physiopathology
- Male -
- Membrane Glycoproteins - deficiency Membrane Glycoproteins - genetics
- Mice -
- Mice, Inbred C57BL -
- Mice, Transgenic -
- NADPH Oxidase - deficiency NADPH Oxidase - genetics
- Nitric Oxide Synthase - deficiency Nitric Oxide Synthase - genetics Nitric Oxide Synthase - immunology
- Platelet Count -
- Reactive Nitrogen Species - immunology
- Reactive Oxygen Species - immunology
- Sepsis - complications Sepsis - immunology Sepsis - physiopathology
- Superoxide Dismutase - genetics
- Find related publications in this database (Keywords)
- Nitric oxide
- NADPH oxidase
- iNOS
- eNOS
- nNOS
- SOD