Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wagner, T; Oberkanins, C; Weinmayr, B; Helmberg, W; Kury, F; Lanzer, G.
HLA-B*27 typing by group-specific hybridization in microtiter plates.
Tissue Antigens. 1998; 52(2):175-178
Doi: 10.1111/j.1399-0039.1998.tb02281.x
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Wagner Thomas
- Co-Autor*innen der Med Uni Graz
- Helmberg Wolfgang
- Lanzer Gerhard
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- Abstract:
- We have developed and evaluated a test for HLA-B*27 based on PCR and DNA hybridization in microtiter plates. A region within exon 2 of the HLA-B gene is amplified and labeled by PCR and the amplification product is hybridized to a group-specific HLA-B*27 and a generic control oligonucleotide probe in two separate cavities of the plate. Bound sequences are detected using an ELISA-like protocol. The assay has been evaluated on 254 DNA samples routinely received for B27 testing in parallel with serological and SSP-PCR typing. Results were concordant in typing 102 HLA-B27-positive and 152 HLA-B27-negative individuals except for two samples containing HLA-B*73, which stained B27 positive in the microwell test. The new procedure is rapid and simple to perform, and the microwell format is particularly suitable for automation.
- Find related publications in this database (using NLM MeSH Indexing)
- Enzyme-Linked Immunosorbent Assay - instrumentation
- HLA-B27 Antigen - analysis
- Histocompatibility Antigens Class I - genetics
- Humans - genetics
- Nucleic Acid Hybridization - genetics
- Oligonucleotide Probes - genetics
- Polymerase Chain Reaction - methods
- Robotics - methods
- Find related publications in this database (Keywords)
- Genetic Typing
- HLA Class I
- HLA-B-Asterisk-27
- Microliter Plates
- NIH Test
- Spondyloarthropathies