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SHR Neuro Cancer Cardio Lipid Metab Microb
Beuchat, A; Thévenaz, P; Unser, M; Ebner, T; Senn, A; Urner, F; Germond, M; Sorzano, CO.
Quantitative morphometrical characterization of human pronuclear zygotes.
Hum Reprod. 2008; 23(9):1983-1992
Doi: 10.1093/humrep/den206
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- Ebner Thomas
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- Abstract:
- BACKGROUND: Identification of embryos with high implantation potential remains a challenge in in vitro fertilization (IVF). Subjective pronuclear (PN) zygote scoring systems have been developed for that purpose. The aim of this work was to provide a software tool that enables objective measuring of morphological characteristics of the human PN zygote. METHODS: A computer program was created to analyse zygote images semi-automatically, providing precise morphological measurements. The accuracy of this approach was first validated by comparing zygotes from two different IVF centres with computer-assisted measurements or subjective scoring. Computer-assisted measurement and subjective scoring were then compared for their ability to classify zygotes with high and low implantation probability by using a linear discriminant analysis. RESULTS: Zygote images coming from the two IVF centres were analysed with the software, resulting in a series of precise measurements of 24 variables. Using subjective scoring, the cytoplasmic halo was the only feature which was significantly different between the two IVF centres. Computer-assisted measurements revealed significant differences between centres in PN centring, PN proximity, cytoplasmic halo and features related to nucleolar precursor bodies distribution. The zygote classification error achieved with the computer-assisted measurements (0.363) was slightly inferior to that of the subjective ones (0.393). CONCLUSIONS: A precise and objective characterization of the morphology of human PN zygotes can be achieved by the use of an advanced image analysis tool. This computer-assisted analysis allows for a better morphological characterization of human zygotes and can be used for classification.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Embryo Implantation - physiology
- Female -
- Humans -
- Image Processing, Computer-Assisted - methods
- Software -
- Zygote - ultrastructure
- Zygote Intrafallopian Transfer -
- Find related publications in this database (Keywords)
- zygote
- in vitro fertilization
- image analysis
- morphometrical characterization