Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Ebner, T; Balaban, B; Moser, M; Shebl, O; Urman, B; Ata, B; Tews, G.
Automatic user-independent zona pellucida imaging at the oocyte stage allows for the prediction of preimplantation development.
Fertil Steril. 2010; 94(3):913-920
Doi: 10.1016/j.fertnstert.2009.03.106
Web of Science
PubMed
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- Führende Autor*innen der Med Uni Graz
- Ebner Thomas
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- Abstract:
- Objective: To analyze whether a change in three-dimensional structure of the zona pellucida could indicate suboptimal gamete quality. Design: Prospective study. Setting: Women's general hospital. Patient(s): A total of 72 patients who gave informed consent. Intervention(s): The birefringence of all oocytes was prospectively analyzed with an automatic user-independent polarization microscopy imaging system. Main Outcome Measure(s): Birefringence of the inner zona layer, preimplantation development, implantation, and pregnancy. Result(s): In approximately one third of all gametes (244/712), the system's automatic detection of the inner zona layer did not succeed. This phenomenon was a negative predictor of compaction (P < 0.01), blastulation (P < 0.001), and pregnancy (P < 0.001). In cases of successful zona imaging, the score based on the birefringence of the inner zona layer was a strong predictor of blastocyst formation but not of embryo quality or pregnancy (P > 0.05). Interestingly, antagonist protocol resulted in lower zona scores as compared with the long protocol (P < 0.05). Conclusion(s): Combining the information from both undetected and detected oocytes, zona imaging was a helpful tool in oocyte selection. This knowledge might further help to reduce both the time in culture and the number of concepti considered for transfer. (Fertil Steril (R) 2010; 94: 913-20. (c) 2010 by American Society for Reproductive Medicine.)
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Algorithms -
- Automation -
- Cleavage Stage, Ovum - cytology Cleavage Stage, Ovum - ultrastructure
- Embryonic Development - physiology
- Female -
- Humans -
- Imaging, Three-Dimensional - methods
- Infertility - diagnosis Infertility - therapy
- Male -
- Microscopy, Polarization - methods
- Oocytes - cytology Oocytes - physiology Oocytes - ultrastructure
- Pregnancy -
- Pregnancy Tests - methods
- Prognosis -
- Software -
- Sperm Injections, Intracytoplasmic -
- Treatment Failure -
- User-Computer Interface -
- Zona Pellucida - ultrastructure
- Find related publications in this database (Keywords)
- Birefringence
- inner layer
- oocyte quality
- polarization microscopy
- zona pellucida
- zona score