Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Corn, CM; Hauser-Kronberger, C; Moser, M; Tews, G; Ebner, T.
Predictive value of cumulus cell apoptosis with regard to blastocyst development of corresponding gametes.
Fertil Steril. 2005; 84(3):627-633
Doi: 10.1016/j.fertnstert.2005.03.061
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Ebner Thomas
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Objective:,To test if a high degree of apoptosis in cumulus cells could indicate diminished oocyte quality and developmental competence Design: Prospective analysis. Setting: Public hospital and university. Patient(s): Thirty seven women who gave written consent to participate in this study. Intervention(s): Cumulus-oocyte complexes were denuded separately and the resulting cumulus cell suspensions were analyzed for presence of apoptosis using a terminal deoxynucleotidyl transferase-mediated digoxigenim-dUTP nick-end labeling (TUNEL) assay detection kit. Main Outcome Measure(s): Percentage of apoptotic cumulus cells and blastocyst formation rate. Result(s): Younger patients (<= 35 years) showed significantly fewer apoptotic cumulus cells than older ones. In all patients gamete maturity was shown to be highly correlated to the rate of apoptosis in cumulus cells. At zygote and cleavage stages (days 1 to 4) no morphologic features were related to the degree of programmed cell death. However,, blastocyst development was predictable taking into account the percentage of apoptosis in associated somatic cells. In addition, there was a trend toward better quality blastocysts from follicles with a lower rate of apoptotic cells. No influence on pregnancy and implantation rate was observed. Conclusion(s): Apoptotic processes within follicles seem to impair oocyte maturation. Though not manifested in the morphologic appearance, gametes and embryos derived from cumulus complexes with no or minor apoptosis have an increased chance of giving rise to optimal blastocysts.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Apoptosis - physiology
- Blastocyst - cytology Blastocyst - physiology
- Cells, Cultured -
- Embryonic Development - physiology
- Female -
- Germ Cells - cytology Germ Cells - physiology
- Humans -
- Oocytes - cytology Oocytes - physiology
- Ovarian Follicle - cytology Ovarian Follicle - physiology
- Predictive Value of Tests -
- Pregnancy -
- Prospective Studies -
- Find related publications in this database (Keywords)
- apoptosis
- blastocyst development
- cumulus cells
- oocyte maturity
- TUNEL assay