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SHR Neuro Cancer Cardio Lipid Metab Microb
Ebner, T; Moser, M; Sommergruber, M; Jesacher, K; Tews, G.
Complete oocyte activation failure after ICSI can be overcome by a modified injection technique.
Hum Reprod. 2004; 19(8): 1837-1841.
Doi: 10.1093/humrep/deh325
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- Ebner Thomas
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- Abstract:
- BACKGROUND: Complete fertilization failure after ICSI is a rare event, and it may happen repeatedly even in cases of normal sperm parameters and good ovarian response. In these cycles, alternative ICSI techniques may prove useful. METHODS: Our modified ICSI (mICSI) is characterized by aspiration close to the opposite membrane (the region of the mitochondria with a high inner mitochondrial membrane potential) which is followed by central deposition of the sperm. The method was applied prospectively to ICSI cycles of patients with a history of complete fertilization failure in previous ICSI cycles. In parallel, mICSI was compared with conventional ICSI in terms of further preimplantation development and treatment outcome. RESULTS: In patients with previous ICSI failures using conventional ICSI (no 2Pn zygotes out of 70 oocytes that had been injected) application of mICSI led to adequate fertilization (53.6%) and pregnancy rates (33.3%) (PANDlt;0.001; PANDlt;0.01). In patients without previous failed fertilization, no improvement in the rates of fertilization, blastocyst formation, implantation or clinical pregnancy could be seen. CONCLUSIONS: Our data indicate that the present version of ICSI is a reliable alternative to conventional ICSI. However, although it overcomes oocyte-dependent activation failure, routine application does not improve the overall results.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Female -
- Fertilization -
- Humans -
- Infertility, Male -
- Male -
- Microinjections - methods
- Mitochondria - physiology
- Oocytes - physiology
- Pregnancy -
- Pregnancy Rate -
- Prospective Studies -
- Sperm Injections, Intracytoplasmic - methods
- Zygote - physiology
- Find related publications in this database (Keywords)
- cytoplasmic maturation
- fertilization failure
- mitochondria
- mitochondrial membrane potential
- oocyte activation