Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Viertler, C; Groelz, D; Gündisch, S; Kashofer, K; Reischauer, B; Riegman, PH; Winther, R; Wyrich, R; Becker, KF; Oelmüller, U; Zatloukal, K.
A New Technology for Stabilization of Biomolecules in Tissues for Combined Histological and Molecular Analyses.
J Mol Diagn. 2012; 14(5):458-466
Doi: 10.1016/j.jmoldx.2012.05.002
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- Führende Autor*innen der Med Uni Graz
- Viertler Christian
- Zatloukal Kurt
- Co-Autor*innen der Med Uni Graz
- Kashofer Karl
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- Abstract:
- For accurate diagnosis, prediction of outcome, and selection of appropriate therapies, the molecular characterization of human diseases requires analysis of a broad spectrum of altered biomolecules, in addition to morphological features, in affected tissues such as tumors. In a high-throughput screening approach, we have developed the PAXgene Tissue System as a novel tissue stabilization technology. Comprehensive characterization of this technology in stabilized and paraffin-embedded human tissues and comparison with snap-frozen tissues revealed excellent preservation of morphology and antigenicity, as well as outstanding integrity of nucleic acids (genomic DNA, miRNA, and mRNA) and phosphoproteins. Importantly, PAXgene-fixed, paraffin-embedded tissues provided RNA quantity and quality not only significantly better than that obtained with neutral buffered formalin, but also similar to that from snap-frozen tissue, which currently represents the gold standard for molecular analyses. The PAXgene tissue stabilization system thus opens new opportunities in a variety of molecular diagnostic and research applications in which the collection of snap-frozen tissue is not feasible for medical, logistic, or ethical reasons. Furthermore, this technology allows performing histopathological analyses together with molecular studies in a single sample, which markedly facilitates direct correlation of morphological disease phenotypes with alterations of nucleic acids and other biomolecules.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Humans -
- Nucleic Acids - metabolism
- Proteins - metabolism
- Specimen Handling - methods
- Tissue Preservation - methods