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SHR Neuro Cancer Cardio Lipid Metab Microb
Iro, M; Klein, R; Gálos, B; Baranyi, U; Rössler, N; Witte, A.
The lysogenic region of virus phiCh1: identification of a repressor-operator system and determination of its activity in halophilic Archaea.
Extremophiles. 2007; 11(2): 383-396.
Doi: 10.1007/s00792-006-0040-3
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- Co-authors Med Uni Graz
- Baranyi Ulrike
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- Abstract:
- phiCh1 is a temperate virus infecting the haloalkaliphilic archaeon Natrialba magadii. As for all temperate viruses, a control of the lysogenic state versus the lytic life cycle is essential. Two open reading frames (ORFs) have been identified as putative repressor encoding genes: ORF48 and ORF49. The protein of ORF48 showed sequence similarities to putative repressor molecules. ORF49 was identified by the analysis of a mutant of phiCh1: the lysogenic strain carrying mutant phiCh1-1 showed a different lysis behavior than wild type virus phiCh1, indicating a dysfunction in the regulation of gene expression. Here, we show that the intergenic region between ORF48 and ORF49 comprises a promoter/operator sequence that is a transcriptionally active region in the model system Haloferax volcanii. Transcription from this region can be repressed by the activity of the ORF48 gene product. Gp43/gp44 has an enhancing effect on this regulatory sequence. Evidence is given for a possible binding site of Rep and gp43/gp44 within the coding region of the rep gene.
- Find related publications in this database (using NLM MeSH Indexing)
- Archaeal Viruses - genetics Archaeal Viruses - metabolism
- Gene Expression Regulation, Viral - physiology
- Halobacteriales - genetics Halobacteriales - virology
- Lysogeny - genetics
- Mutation -
- Open Reading Frames -
- Operator Regions, Genetic -
- Promoter Regions, Genetic -
- Repressor Proteins - genetics
- Transcription, Genetic - physiology
- Viral Proteins - genetics Viral Proteins - metabolism
- Find related publications in this database (Keywords)
- Natrialba
- gene regulation
- haloalkaliphilic
- reporter gene
- halovirus