Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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Malvasio, V; Ainoedhofer, H; Ackbar, R; Hoellwarth, ME; Saxena, AK.
Effects of sodium hydroxide exposure on esophageal epithelial cells in an in vitro ovine model: implications for esophagus tissue engineering.
J Pediatr Surg. 2012; 47(5):874-880
Doi: 10.1016/j.jpedsurg.2012.01.033
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Malvasio Valeria
- Saxena Amulya Kumar
- Co-Autor*innen der Med Uni Graz
- Ackbar Richard
- Ainödhofer Herwig
- Höllwarth Michael
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- Abstract:
- Background: Esophagus tissue engineering holds promises for esophageal replacement after severe caustic injuries. The aim of this study was to determine whether viable esophageal epithelial cells could be isolated from an esophagus exposed to varying concentrations of alkali with regard to number, viability, and morphology during in vitro culture. Methods: Ovine esophagi were exposed to phosphate-buffered saline 2.5%, 15%, or 25% sodium hydroxide (NaOH). The effect of NaOH concentrations on epithelial damage was assessed histologically. Esophageal epithelial cells were then isolated, and cell count and viability were investigated. Finally, cell number, viability, and morphology of esophageal epithelial cells were determined for 24 days of in vitro culture. Results: Histologic analysis showed a progressive destruction of the epithelium proportional to increasing NaOH concentrations. Esophagi treated with phosphate-buffered saline and 2.5% NaOH showed significantly higher viable cell counts after isolation and culture in comparison with those treated with 15% to 5% NaOH. Conclusion: The evidence presented in this study indicates that epithelial biopsies from an esophagus exposed to low concentrations (2.5%) of NaOH will still yield large numbers of viable cells suitable for tissue engineering applications. In cases of exposure to higher concentrations (15%-25%), alternative cell sources for epithelial regeneration, such as stem cells, will be necessary for tissue engineering applications. (c) 2012 Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Biopsy -
- Caustics - toxicity
- Cell Count -
- Cell Survival -
- Cells, Cultured -
- Dose-Response Relationship, Drug -
- Epithelial Cells - drug effects Epithelial Cells - pathology Epithelial Cells - physiology
- Esophagus - drug effects Esophagus - pathology
- Sheep -
- Sodium Hydroxide - toxicity
- Tissue Engineering -
- Find related publications in this database (Keywords)
- Esophageal epithelial cells
- Caustic injury
- Sodium hydroxide
- Culture
- Tissue engineering