Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kamruzzahan, AS; Kienberger, F; Stroh, CM; Berg, J; Huss, R; Ebner, A; Zhu, R; Rankl, C; Gruber, HJ; Hinterdorfer, P.
Imaging morphological details and pathological differences of red blood cells using tapping-mode AFM.
Biol Chem. 2004; 385(10):955-960
Doi: 10.1515/BC.2004.124
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Berg Jörg
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- Abstract:
- The surface topography of red blood cells (RBCs) was investigated under near-physiological conditions using atomic force microscopy (AFM). An immobilization protocol was established where RBCs are coupled via molecular bonds of the membrane glycoproteins to wheat germ agglutinin (WGA), which is covalently and flexibly tethered to the support. This results in a tight but non-invasive attachment of the cells. Using tapping-mode AFM, which is known as gentle imaging mode and therefore most appropriate for soft biological samples like erythrocytes, it was possible to resolve membrane skeleton structures without major distortions or deformations of the cell surface. Significant differences in the morphology of RBCs from healthy humans and patients with systemic lupus erythematosus (SLE) were observed on topographical images. The surface of RBCs from SLE patients showed characteristic circular-shaped holes with approx. 200 nm in diameter under physiological conditions, a possible morphological correlate to previously published changes in the SLE erythrocyte membrane.
- Find related publications in this database (using NLM MeSH Indexing)
- Erythrocyte Membrane - metabolism Erythrocyte Membrane - pathology Erythrocyte Membrane - ultrastructure
- Erythrocytes - metabolism Erythrocytes - pathology Erythrocytes - ultrastructure
- Humans -
- Lupus Erythematosus, Systemic - metabolism Lupus Erythematosus, Systemic - pathology
- Microscopy, Atomic Force - methods
- Surface Properties -
- Find related publications in this database (Keywords)
- AFM
- erythrocytes
- imaging
- molecular recognition
- red blood cells
- SLE