Medizinische Universität Graz - Research portal
Selected Publication:
Berg, J; Doe, B; Steimer, KS; Wabl, M.
HeLa-LAV, an epithelial cell line stably infected with HIV-1.
J Virol Methods. 1991; 34(2):173-180
Doi: 10.1016/0166-0934(91)90097-J
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Leading authors Med Uni Graz
- Berg Jörg
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- An HeLa-LAV cell line was established by infecting and subcloning previously described CD4-expressing HeLa cells with HIV-1. Cells of this line stably synthesize all major HIV proteins, release infectious particles of HIV-1, but do not die even after long term culture. More than 90% of the cells express the envelope protein gp120 on the surface. The cells can be easily and efficiently labeled with 51chromium, and exhibit a low spontaneous release. Because they are susceptible to killing by allogeneic cytotoxic T cells (CTL) when targeted to gp120, they ought to be a useful source of target cells in any kind of HIV-specific killing assays. The cells may also help studies on HIV replication in non-lymphatic/non-monocytic cells. The HeLa-LAV cell line will be freely available from the AIDS Research and Reference Reagent Program.
- Find related publications in this database (using NLM MeSH Indexing)
- CD4-Positive T-Lymphocytes - immunology
- Cell Line -
- Cells, Cultured -
- Clone Cells -
- Cytotoxicity, Immunologic -
- Electrophoresis, Polyacrylamide Gel -
- Fluorescent Antibody Technique -
- Gene Products, gag - analysis
- HIV Envelope Protein gp120 - analysis
- HIV-1 -
- HeLa Cells - immunology HeLa Cells - microbiology
- Humans -
- T-Lymphocytes, Cytotoxic - immunology
- gag Gene Products, Human Immunodeficiency Virus -
- Find related publications in this database (Keywords)
- HELA-LAV CELL
- HELAT4+ CELL
- HIV-1 INFECTION
- CHROMIUM-51 RELEASE ASSAY