Medizinische Universität Graz - Research portal
Selected Publication:
Maly, K; Hochleitner, B; Uberall, F; Loferer, H; Oberhuber, H; Doppler, W; Grunicke, H.
Mechanism and biological significance of the Ha-ras-induced activation of the Na+/H(+)-antiporter.
Adv Enzyme Regul. 1990; 30(3):63-74
Doi: 10.1016/0065-2571(90)90009-Q
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Co-authors Med Uni Graz
- Hager Boris
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Expression of the transforming Ha-ras oncogene in MMTV-LTR transfected NIH 3T3 cells leads to a growth factor independent activation of the Na+/H(+)-antiporter. The activation of the antiporter is insensitive to the protein kinase inhibitor staurosporine and equally expressed in protein kinase C-depleted cells. It is concluded that the Ha-ras induced activation of the antiporter occurs by a protein kinase C-independent mechanism. An inhibition of the Na+/H(+)-antiporter by dimethylamiloride or a reduction of the extracellular [Na+] concentration results in a depression of the bombesin induced release of Ca2+ from intracellular stores. These results are explained by a steep pH-dependence of the Ca2(+)-mobilizing system which exhibits a maximum at pH 7.1 in the system studied here. Stimulation by growth factors of quiescent cells with a resting pH below 7 results in a shift of the cytosolic pH towards the optimum for the Ca2+ release. In agreement with the proposed interrelationship, pHi and [Ca2+]i rise and peak simultaneously after addition of bombesin to G0 arrested cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Alkaloids - pharmacology
- Animals -
- Bombesin - pharmacology
- Calcium - metabolism
- Carrier Proteins - metabolism
- Cell Line -
- Cytosol - metabolism
- Dexamethasone - pharmacology
- Genes, ras -
- Hydrogen-Ion Concentration -
- Kinetics -
- Mammary Tumor Virus, Mouse - genetics
- Mice -
- Protein Kinase C - antagonists and inhibitors
- Sodium-Hydrogen Antiporter -
- Staurosporine -
- Tetradecanoylphorbol Acetate - pharmacology
- Transfection -