Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Robier, C; Quehenberger, F; Neubauer, M; Stettin, M; Rainer, F.
Pseudoeosinophilia of synovial fluid is caused by crystals mimicking the distinct light scattering fractions of eosinophilic granules.
Clin Chem Lab Med. 2012; 50(2):371-373
Doi: 10.1515/CCLM.2011.745
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Robier Christoph
- Co-Autor*innen der Med Uni Graz
- Quehenberger Franz
- Stettin Mariana
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- Abstract:
- Background: Automated leukocyte differential counts of synovial fluid (SF) can be influenced by laboratory artefacts. Pseudoeosinophilia of SF has recently been first described in association with monosodium urate and calcium pyrophosphate crystals. This study compared automated measurements of the percentages of SF leukocyte fractions by two haematology analysers in order to elucidate the underlying mechanism of pseudoeosinophilia. Methods: The percentages of the leukocyte fractions of 17 crystal-containing and 28 crystal-free specimens were compared using the Wilcoxon test. Measurements were performed using the Cell-Dyn 3200 and the ADVIA 2120i, which are based on different techniques. Results: The percentages of eosinophils of the crystal-positive samples determined by the Cell-Dyn 3200 were significantly higher than those assessed by the ADVIA (p<0.0001), whereas the percentages of eosinophils of the controls did not differ significantly between the two devices (p=0.95). The Cell-Dyn 3200 clearly showed the phenomenon of crystal-associated pseudoeosinophilia (p<0.0001), which did not occur in the ADVIA measurements (p=0.28). The percentage of neutrophils was to a lower degree elevated in the crystal group (p=0.015). Conclusions: It was confirmed that SF crystals interfere with the typical light scattering fractions of leukocyte granules and may thus lead to spuriously elevated percentages of eosinophils and neutrophils in SF specimens.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Aged, 80 and over -
- Calcium Pyrophosphate - chemistry
- Eosinophilia - diagnosis
- Eosinophils - chemistry
- Female -
- Humans -
- Male -
- Microscopy -
- Middle Aged -
- Reference Standards -
- Reproducibility of Results -
- Synovial Fluid - chemistry
- Uric Acid - chemistry
- Find related publications in this database (Keywords)
- calcium pyrophosphate
- eosinophilia
- monosodium urate
- pseudoeosinophilia
- synovial fluid