Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Schernthaner, M; Reisinger, B; Wolinski, H; Kohlwein, SD; Trantina-Yates, A; Fahrner, M; Romanin, C; Itani, H; Stifter, D; Leitinger, G; Groschner, K; Heitz, J.
Nanopatterned polymer substrates promote endothelial proliferation by initiation of β-catenin transcriptional signaling.
Acta Biomater. 2012; 8(8):2953-2962
Doi: 10.1016/j.actbio.2012.04.018
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Groschner Klaus
- Schernthaner Michaela
- Co-Autor*innen der Med Uni Graz
- Leitinger Gerd
- Yates Ameli
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- Abstract:
- Control of endothelial phenotype involves a variety of signaling pathways and transcriptional regulators, including the junctional protein β-catenin. This multifunctional signaling molecule is part of adhesion contacts in the endothelium and is able to translocate into the nucleus to activate genetic programs and control proliferation and the fate of the cells. We investigated the influence of laser-generated nanopatterns on polymeric cell culture substrates on endothelial tissue architecture, proliferation and β-catenin signaling. For our experiments human microvascular endothelial cells or CD34(+) endothelial progenitor cells, isolated from human adipose tissue, were cultured on polyethylene terephthalate (PET) substrates with oriented nanostructures with lateral periodicities of 1.5 μm and 300 nm, respectively. The surface topography and chemistry of the PET substrates were characterized by electron microscopy, atomic force microscopy, water contact angle measurement and X-ray photoelectron spectroscopy. Analysis of cell phenotype markers as well as β-catenin signaling revealed that short-term culture of endothelial cells on nanostructured substrates generates a proliferative cell phenotype associated with nuclear accumulation of β-catenin and activation of specific β-catenin target genes. The effects of the nanostructures were not directly correlated with nanostructure-induced alignment of cells and were also clearly distinguishable from the effects of altered PET surface chemistry due to photomodification. In summary, we present a novel mechanism of surface topology-dependent control of transcriptional programs in mature endothelium and endothelial progenitor cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Adhesion - drug effects
- Cell Proliferation - drug effects
- Cyclin D1 - metabolism
- Endothelial Cells - cytology
- Humans -
- Microscopy, Atomic Force -
- Microvessels - cytology
- Nanostructures - chemistry
- Photoelectron Spectroscopy -
- Polyethylene Terephthalates - chemistry
- Protein Transport - drug effects
- Signal Transduction - drug effects
- Transcription, Genetic - drug effects
- Water - chemistry
- beta Catenin - genetics
- Find related publications in this database (Keywords)
- Endothelial cell
- Cell signaling
- Laser manufacturing
- Nanotopography