Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Krenn, EC; Wille, I; Gesslbauer, B; Poteser, M; van, Kuppevelt, TH; Kungl, AJ.
Glycanogenomics: a qPCR-approach to investigate biological glycan function.
Biochem Biophys Res Commun. 2008; 375(3):297-302 Doi: 10.1016/j.bbrc.2008.07.144
Web of Science PubMed FullText FullText_MUG

Leading authors Med Uni Graz: Jantscher-Krenn Evelyn
Co-authors Med Uni Graz: Poteser Michael
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: As an indirect approach towards glycan structures, qRT-PCR analyses using the DeltaDeltaC(T) method were performed to investigate changes in expression levels of heparan sulfate-synthesising enzymes of stimulated and unstimulated HMVECs. We chose NDSTs as early enzymes initiating sulfation and 3OSTs which act late generating specific binding sites. Major changes in expression patterns were found for the NDST3 and 3OST1 isoforms. Both enzymes were down-regulated 7- and 6-fold, respectively, following TNF-alpha stimulation, and 3.5- and 7.6-fold following LPS-stimulation suggesting a common restructuring process of HS in inflammation leading to a less diverse sulfation pattern. Immunostaining of TNF-alpha-stimulated cells using a phage display-derived antibody specific for 3-O-sulfation and unsulfated regions of HS resulted in significant fluorescence changes between unstimulated and stimulated.
Find related publications in this database (using NLM MeSH Indexing): Cell Line - administration & dosage; Endothelium, Vascular - drug effects, enzymology, metabolism; Genomics - methods; Heparitin Sulfate - biosynthesis, genetics; Humans - administration & dosage; Polymerase Chain Reaction - methods; Polysaccharides - biosynthesis, genetics; Sulfotransferases - genetics; Tumor Necrosis Factor-alpha - pharmacology
Find related publications in this database (Keywords): heparan sulfate; endothelium; inflammation; TNF-alpha; N-deacetylase/N-sulfotransferase; 3-O-sulfotransferase; real-time PCR