Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Chin, A; Svejda, B; Gustafsson, BI; Granlund, AB; Sandvik, AK; Timberlake, A; Sumpio, B; Pfragner, R; Modlin, IM; Kidd, M.
The role of mechanical forces and adenosine in the regulation of intestinal enterochromaffin cell serotonin secretion.
AM J PHYSIOL-GASTR L. 2012; 302(3): G397-G405.
Doi: 10.1152/ajpgi.00087.2011
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Pfragner Roswitha
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Enterochromaffin (EC) cells of the diffuse neuroendocrine cell system secrete serotonin (5-HT) with activation of gut motility, secretion, and pain. These cells express adenosine (ADORA) receptors and are considered to function as mechanosensors. Physiological pathways mediating mechanosensitivity and adenosine responsiveness remain to be fully elucidated, as do their roles in inflammatory bowel disease (IBD) and neoplasia. Pure (98-99%) FACS-sorted normal and IBD human EC cells and neoplastic EC cells (KRJ-I) were studied. IBD-EC cells and KRJ-I overexpressed ADORA2B. NECA, a general ADORA receptor agonist, stimulated, whereas the A2B receptor antagonist MRS1754 inhibited, 5-HT release (EC50 = 1.8 Ã 10-6 M; IC50 = 3.7 Ã 10-8 M), which was associated with corresponding alterations in intracellular cAMP levels and pCREB (Ser133). Mechanical stimulation using a rhythmic flex model induced transcription and activation of Tph1 (tryptophan hydroxylase) and VMATâ (vesicular monoamine transporter 1) and the release of 5-HT, which could be inhibited by MRS1754 and amplified by NECA. Secretion was also inhibited by H-89 (PKA inhibitor) while Tph1 and VMATâ transcription was regulated by PKA/MAPK and PIâK-mediated signaling. Normal and IBD-EC cells also responded to NECA and mechanical stimulation with PKA activation, cAMP production, and 5-HT release, effects reversible by MRS1754. EC cells express stimulatory ADORA2B, and rhythmic stretch induces A2B activation, PKA/MAPK/IP3-dependent transcription, and PKA-dependent secretion of 5-HT synthesis and secretion. Receptor expression is amplified in IBD and neoplasia, and 5-HT release is increased. Determination of factors that regulate EC cell function are necessary for understanding its role as a mechanosensory cell and to facilitate the development of agents that can selectively target cell function in EC cell-associated disease.
- Find related publications in this database (using NLM MeSH Indexing)
- Acetamides - pharmacology
- Adenosine - pharmacology
- Adenosine A2 Receptor Agonists - pharmacology
- Adenosine A2 Receptor Antagonists - pharmacology
- Adenosine-5'-(N-ethylcarboxamide) - pharmacology
- Adult -
- Aged -
- Cell Line, Tumor -
- Cells, Cultured -
- Colon - cytology
- Crohn Disease - metabolism
- Cyclic AMP - metabolism
- Cyclic AMP Response Element-Binding Protein - metabolism
- Cyclic AMP-Dependent Protein Kinases - antagonists and inhibitors
- Enterochromaffin Cells - drug effects
- Female -
- Gene Expression - genetics
- Humans -
- MAP Kinase Kinase 1 - antagonists and inhibitors
- MAP Kinase Signaling System - drug effects
- Male -
- Mechanotransduction, Cellular - drug effects
- Middle Aged -
- Phosphorylation - drug effects
- Proto-Oncogene Proteins c-akt - antagonists and inhibitors
- Purines - pharmacology
- Receptor, Adenosine A1 - genetics
- Receptor, Adenosine A2A - genetics
- Receptor, Adenosine A2B - genetics
- Receptor, Adenosine A3 - genetics
- Serotonin - secretion
- Signal Transduction - drug effects
- Stress, Mechanical -
- Tryptophan Hydroxylase - genetics
- Vesicular Monoamine Transport Proteins - metabolism
- Find related publications in this database (Keywords)
- adenosine
- Crohn's disease
- enterochromaffin cell
- serotonin